FLI Specificity Experiment:
Experimental NIRF probe: ICG-like
Probe max Ex/Em = 710/790 nm
Test Mice (n=3): Received ICG-like injection, IP
All mice imaged immediately after IP injection
FLI Specificity Experiment:
NIRF probe: OsteosenseTM 680; targets hydroxyapatite in growth plates of long bones
Probe max Ex/Em = 668/687 nm
Test Mice (n=2): 100uL Osteosense™680, IP
Control mice (n=2): 100 uL PBS, IP
All mice imaged 24 hrs post IP injection
Orientation during imaging: Mice 1, 3: dorsal | Mice 2, 4: ventral
LED spectrums are inherently narrow and specific – falling within the 20 nm filter bandwidth
White light has a broad emission and relies on filters to limit 93.5% of light. Out-of-band photons result from the physical limitations of filters blocking excessive light.
Conclusion:
Out-of-band, excitation-based light is detected across a range of Em filters in white light based competitors. This non-specific, out-of-band light, reflects off mice, reducing Signal/Noise ratio.
Use multiple probes to answer complex biological questions. Find out how our patented LED technology can help.
Individually controlled LED’s allow you to avoid overlapping fluorescent spectra entirely – providing clean, multiplex fluorescent data.
Continuous light from white tungsten bulb requires software algorithms to artificially separate spectra.
A multiple gas condensation system. Slow, noisy, and prone to failure
Faster, but liquid plumbing still susceptible to leaks
No leaks, fast cooling down to -90˚C in 5 minutes
BLI Sensitivity Experiment:
Luciferase probe: Human pancreatic cell line S2VP10
All mice received IP injection of 2.5 mg luciferin 10 min prior to imaging
Allows you to easily image 5 mice in a single exposure
Courtesy of Dr. Jun Wu | Stanford University School of Medicine
Ami HTX
*Representing Leading Competitor FOV
Sharon S. Hori, Sheen-Woo Lee, Sanjiv Sam Gambhir (Canary Center at Stanford, Stanford University School of Medicine)
Spectral Instruments Imaging is the fusion of principal developers in in vivo imaging technology and a world class camera manufacturer. Working together to advance pre-clinical imaging to reveal complex biological processes.
We were so pleased with the instrument’s performance and SII’s support within the very first year of use that we committed to a replacement of our entire suite of older instruments with SII products.
The LED based illumination meant that there was nearly a 90X more light incident on the surface of the specimen versus traditional white lights used by other manufacturers which translates to earlier detection – sometimes weeks ahead – saving researchers time and money. It also dramatically improved the utilization of the imaging core.
SII has a single-minded focus on building the best optical imaging instruments for bioluminescence and fluorescence. The entire company – management, engineers, sales team and support is aligned around that central focus.
This has meant many good things for us as a customer – where the innovation continues non-stop. We are already seeing many new features in software and hardware that leave us very optimistic about the future.
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