FLI Specificity Experiment:
Experimental NIRF probe: ICG-like
Probe max Ex/Em = 710/790 nm
Test Mice (n=3): Received ICG-like injection, IP
All mice imaged immediately after IP injection
FLI Specificity Experiment:
NIRF probe: OsteosenseTM 680; targets hydroxyapatite in growth plates of long bones
Probe max Ex/Em = 668/687 nm
Test Mice (n=2): 100uL Osteosense™680, IP
Control mice (n=2): 100 uL PBS, IP
All mice imaged 24 hrs post IP injection
Orientation during imaging: Mice 1, 3: dorsal | Mice 2, 4: ventral
The physics behind the data:
LED spectrums are inherently narrow and specific – falling within the 20 nm filter bandwidth
White light has a broad emission and relies on filters to limit 93.5% of light. Out-of-band photons result from the physical limitations of filters blocking excessive light.
Conclusion:
Out-of-band, excitation-based light is detected across a range of Em filters in white light based competitors. This non-specific, out-of-band light, reflects off mice, reducing Signal/Noise ratio.
Ready to Start Multiplexing?
Use multiple probes to answer complex biological questions. Find out how our patented LED technology can help.
High Sensitivity + Low Background = Accurate Quantification
Individually controlled LED’s allow you to avoid overlapping fluorescent spectra entirely – providing clean, multiplex fluorescent data.
Continuous light from white tungsten bulb requires software algorithms to artificially separate spectra.
Evolution of -90°C Cooling
CryoTiger:
A multiple gas condensation system. Slow, noisy, and prone to failure
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Faster, but liquid plumbing still susceptible to leaks
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Reaches -90°C in only 5 minutes.
Learn more about the importance of Ultra Cold CCD Cameras
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BLI Sensitivity Experiment:
Luciferase probe: Human pancreatic cell line S2VP10
All mice received IP injection of 2.5 mg luciferin 10 min prior to imaging
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Spectral’s engineering team eliminated
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Less Pieces and Parts to Break = Lower Lifetime Cost of Ownership
High Capacity In vivo Imaging
High Capacity In vivo Imaging Now Available on your Benchtop
Now Available on your Benchtop
Ami HTX FoV 25x17cm
Allows you to easily image 5 mice in a single exposure
Courtesy of Dr. Jun Wu | Stanford University School of Medicine
Ami HTX
Leading Competitor FOV 10x10cm
*Representing Leading Competitor FOV
Experience the Ultimate High Throughput
In vivo imaging system: Lago X
Experience the Ultimate High Throughput In vivo Imaging System: Lago X
Sharon S. Hori, Sheen-Woo Lee, Sanjiv Sam Gambhir (Canary Center at Stanford, Stanford University School of Medicine)
- Designed for those who consider small animal imaging to be their life’s work
- Industry Leading 10 mouse capacity with 25×25 cm FOV
- 14 Wavelengths and 20 Filters means every fluorophore is available without changing a filter for non-stop imaging!
Imagine the Possibilities
Experts trust Experts
Spectral Instruments Imaging is the fusion of principal developers in in vivo imaging technology and a world class camera manufacturer. Working together to advance pre-clinical imaging to reveal complex biological processes.
What researchers are saying...
We were so pleased with the instrument’s performance and SII’s support within the very first year of use that we committed to a replacement of our entire suite of older instruments with SII products.
The LED based illumination meant that there was nearly a 90X more light incident on the surface of the specimen versus traditional white lights used by other manufacturers which translates to earlier detection – sometimes weeks ahead – saving researchers time and money. It also dramatically improved the utilization of the imaging core.
SII has a single-minded focus on building the best optical imaging instruments for bioluminescence and fluorescence. The entire company – management, engineers, sales team and support is aligned around that central focus.
This has meant many good things for us as a customer – where the innovation continues non-stop. We are already seeing many new features in software and hardware that leave us very optimistic about the future.
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