Left: Ami HTX | Right: IVIS® Lumina
FLI Specificity Experiment:
Experimental NIRF probe: ICG-like
Probe max Ex/Em = 710/790 nm
Test Mice (n=3): Received ICG-like injection, IP
All mice imaged immediately after IP injection
Left: Ami HTX – Courtesy of Dr. Timothy Doyle, Molecular Imaging Program at Stanford, Dept. of Pediatrics, School of Medicine, Stanford University
Right: IVIS® Spectrum
FLI Specificity Experiment:
NIRF probe: OsteosenseTM 680; targets hydroxyapatite in growth plates of long bones
Probe max Ex/Em = 668/687 nm
Test Mice (n=2): 100uL Osteosense™680, IP
Control mice (n=2): 100 uL PBS, IP
All mice imaged 24 hrs post IP injection
Orientation during imaging: Mice 1, 3: dorsal | Mice 2, 4: ventral
LED spectrums are inherently narrow and specific – falling within the 20 nm filter bandwidth
White light has a broad emission and relies on filters to limit 93.5% of light. Out-of-band photons result from the physical limitations of filters blocking excessive light.
Out-of-band, excitation-based light is detected across a range of Em filters in white light based competitors. This non-specific, out-of-band light, reflects off mice, reducing Signal/Noise ratio.
Use multiple probes to answer complex biological questions. Find out how our patented LED technology can help.
Individually controlled LED’s allow you to avoid overlapping fluorescent spectra entirely – providing clean, multiplex fluorescent data.
Continuous light from white tungsten bulb requires software algorithms to artificially separate spectra.
A multiple gas condensation system. Slow, noisy, and prone to failure
Faster, but liquid plumbing still susceptible to leaks
No leaks, fast cooling down to -90˚C in 5 minutes
BLI Sensitivity Experiment:
Luciferase probe: Human pancreatic cell line S2VP10
All mice received IP injection of 2.5 mg luciferin 10 min prior to imaging
Courtesy of Dr. Jun Wu | Stanford University School of Medicine
*Representing IVIS® Lumina XRMS
Sharon S. Hori, Sheen-Woo Lee, Sanjiv Sam Gambhir (Canary Center at Stanford, Stanford University School of Medicine)
We were so pleased with the instrument’s performance and SII’s support within the very first year of use that we committed to a replacement of our entire suite of older instruments with SII products.
The LED based illumination meant that there was nearly a 90X more light incident on the surface of the specimen versus traditional white lights used by other manufacturers which translates to earlier detection – sometimes weeks ahead – saving researchers time and money. It also dramatically improved the utilization of the imaging core.
SII has a single-minded focus on building the best optical imaging instruments for bioluminescence and fluorescence. The entire company – management, engineers, sales team and support is aligned around that central focus.
This has meant many good things for us as a customer – where the innovation continues non-stop. We are already seeing many new features in software and hardware that leave us very optimistic about the future.
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