| Qin, X., Zhou, K., Dong, L., Yang, L., Li, W., Chen, Z., Shen, C., Han, L., Li, Y., Chan, A.K.N., Pokharel, S.P., Qing, Y., Chen, M., Wang, K., Leung, K., Sau, L., Chen, C.W., Deng, X., Su, R., Chen, J. (2025). CRISPR screening reveals ZNF217 as a vulnerability in high-risk B-cell acute lymphoblastic leukemia. Theranostics, 15(8), 3234-3256. | Jianjun Chen, Department of Systems Biology, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA | Cancer Biology | Leukemia | BLI | Mouse | Lago X | 2025 | This study identifies ZNF217 as a critical gene for the survival and growth of high-risk B-cell acute lymphoblastic leukemia (B-ALL) subtypes, specifically those with MLL rearrangements or BCR-ABL fusion. Using CRISPR-Cas9 screening, in vitro and in vivo models, and integrative multi-omics analyses, the researchers demonstrated that ZNF217 supports leukemia progression through both CoREST-dependent and independent mechanisms, including repression of the FOS gene. Depleting ZNF217 significantly reduced leukemia progression and improved survival in mouse models, highlighting ZNF217 as a promising therapeutic target for high-risk B-ALL. | | cancer-biology | leukemia |
| Gilbert, B. R., Miglani, C., Karmakar, A., Pal, M., Chandran, V. C., Gupta, S., Pal, A., & Ganguli, M. (2025). A combination of systemic mannitol and mannitol modified polyester nanoparticles for caveolae-mediated gene delivery to the brain. Molecular Therapy Nucleic Acids, 36, 102480. | Munia Ganguli, CSIR-Institute of Genomics and Integrative Biology, Mathura Road, New Delhi 110025, India | Gene Therapy | Engineered Nanoparticles | FLI | Mouse | Lago X | 2025 | This study presents a novel strategy for delivering nucleic acids across the blood-brain barrier (BBB) using a dual approach: mannitol-modified poly(β-amino ester) (PBAE) nanoparticles (M30 D90) and systemic mannitol injection. The mannitol-modified nanoparticles are designed to target caveolae, small invaginations in endothelial cells, while systemic mannitol injection induces caveolae formation at the BBB. This combination enabled effective, selective delivery of plasmid DNA to neuronal cells in the brain, with minimal off-target accumulation in the liver. The approach demonstrates a promising, scalable platform for non-viral gene delivery to the brain. | | gene-therapy | engineered-nanoparticles |
| Hu, X., Tediashvili, G., Gravina, A. et al. Inhibition of polymorphonuclear cells averts cytotoxicity against hypoimmune cells in xenotransplantation. Nat Commun 16, 3706 (2025). | Sonja Schrepfer, Sana Biotechnology Inc., 1 Tower Place, South San Francisco, CA 94080, USA | Regenerative Medicine | Hypoimmune Cell Engineering | BLI | Mouse | Lago | 2025 | This study addresses the challenge of innate immune rejection in xenotransplantation by focusing on immune-evasive cell therapeutics. Researchers engineered human hypoimmune (HIP*) endothelial cells (ECs) for transplantation into non-human primates by removing HLA expression and expressing macaque-compatible CD47. Despite avoiding rejection by T cells, NK cells, macrophages, and antibodies, the human HIP* ECs were still targeted by macaque polymorphonuclear cells (PMNs). A multi-drug regimen successfully suppressed this PMN-mediated killing, improving graft survival. The study extended to pig HIP* ECs, which were modified to evade human immune responses by knocking out SLA and overexpressing human CD47, CD99, and CD200. These cells resisted cytotoxicity from all arms of the human immune system, including PMNs. The findings highlight the importance of targeting PMN responses to enhance the success of xenotransplantation therapies. | | regenerative-medicine | hypoimmune-cell-engineering |
| Smith, S J., Meng, F., Lingeman, R. G., Li, C. M., Li, M., Boneh, G., Seppälä, T. T., Phan, T., Li, H., Burkhart, R. A., Parekh, V., Rahmanuddin, S., Melstrom, L. G., Hickey, R. J., Chung, V., Liu, Y., Malkas, L. H., & Raoof, M. (2025). Therapeutic targeting of oncogene‑induced transcription‑replication conflicts in pancreatic ductal adenocarcinoma. Gastroenterology, 2025 1–15. | Mustafa Raoof, Department of Surgery, City of Hope Cancer Center, Duarte, California USA | Cancer Biology | Pancreatic Ductal Adenocarcinoma | BLI | Mouse | Lago X | 2025 | This study investigates the small molecule AOH1996 as a therapeutic strategy targeting transcription-replication conflicts (TRCs) in pancreatic ductal adenocarcinoma (PDAC), a cancer type with high replication stress. Using KRAS(G12D)-inducible models, PDAC cell lines, organoids, and mouse models, the authors show that AOH1996 causes transcription-dependent DNA damage, inhibits replication fork progression, and selectively kills tumor cells with high TRC burden. AOH1996 was especially effective in PDAC subtypes with elevated replication stress and demonstrated tumor shrinkage and extended survival in preclinical models. Two chemotherapy-refractory PDAC patients treated with AOH1996 showed partial radiographic responses, suggesting early clinical potential. | | cancer-biology | pancreatic-ductal-adenocarcinoma |
| Luostarinen A, Vuorela A, Kerkelä E, et al. Establishing a GMP-compliant manufacturing process and phase-appropriate analytics for early development of a FiCAR T-cell product with a novel CAR spacer. Sci Rep. 2025;15:8093. | Annu Luostarinen, Advanced Cell Therapy Centre, Finnish Red Cross Blood Service, Härkälenkki 13, 01,730, Vantaa, Finland | Cell and Gene Therapy | Chimeric Antigen Receptor (CAR) T Cell Therapy | BLI | Mouse | Lago X | 2025 | This article describes the development of a GMP-compliant manufacturing process and phase-appropriate analytics for a new autologous CD19-targeted CAR T-cell therapy, 19-FiCART, which features a novel SIRPα-based spacer (FiCAR). The team optimized a 12-day semi-automated production process using healthy donor leukapheresis products and found the source material could remain stable for up to 73 hours at 2–8 °C. The process consistently produced sufficient quantities of viable CAR+ T cells with strong anti-tumor activity in vitro and in a xenograft mouse lymphoma model. The paper also details quality control measures and release criteria suitable for early-phase clinical trials and offers insights for establishing local ATMP manufacturing centers." | | cell-and-gene-therapy | chimeric-antigen-receptor-car-t-cell-therapy |
| Hsieh, H.-J., Urak, R., Clark, M. C., Kwak, L. W., Forman, S. J., & Wang, X. (Year). Capivasertib enhances chimeric antigen receptor T cell activity in preclinical models of B cell lymphoma. Molecular Therapy Methods and Clinical Development. 2025. | "Xiuli Wang, Department of Hematology and HCT, City of Hope National Medical Center, Duarte, CA 91010, USA." | Cancer Immunotherapy | Chimeric Antigen Receptor (CAR) T Cell Therapy | BLI | Mouse | Lago X | 2025 | "This study investigates the use of capivasertib, a pan-AKT inhibitor, to enhance the effectiveness of chimeric antigen receptor (CAR) T cell therapy against B cell lymphoma. The PI3K/AKT signaling pathway is critical for both cancer cell growth and T cell activation. The authors previously showed that inhibiting AKT during CAR T cell manufacturing improves T cell function, but the inhibitor used was not clinically suitable. Here, they demonstrate that treating CAR T cells with capivasertib during manufacturing enhances their anti-tumor activity in mouse models. Additionally, combining capivasertib with CD19-targeting CAR T cells in vivo improves early treatment response and persistence of functional CAR T cells, especially in tumors deficient in PTEN—a common cancer mutation that influences AKT signaling. This combined approach holds promise for overcoming some limitations of current CAR T cell therapies.
" | | cancer-immunotherapy | chimeric-antigen-receptor-car-t-cell-therapy |
| Wachsmann TLA, Poortvliet T, Meeuwsen MH, Remst DFG, Toes MF, Wouters AK, Hagedoorn RS, Falkenburg JHF, Heemskerk MHM. CAR-mediated target recognition limits TCR-mediated target recognition of TCR- and CAR-dual-receptor-edited T cells. Molecular Therapy. 2025. | "Tassilo L.A. Wachsmann, Department of Hematology, Leiden
University Medical Center, 2333ZA Leiden, Netherlands" | Cancer Immunotherapy | Chimeric Antigen Receptor (CAR) T Cell Therapy | BLI | Mouse | Lago | 2025 | This study investigates the engineering of CD8 T cells with dual specificity by combining a transgenic T cell receptor (TCR) targeting a BOB1-derived peptide (HLA-B*07:02-restricted) and a chimeric antigen receptor (CAR) targeting BCMA, a common antigen in multiple myeloma (MM). These dual-receptor T cells, called TRaCR T cells, can recognize and kill tumor cells that evade either single targeting strategy alone, showing broad targeting potential. However, in cells expressing both antigens, recognition is dominated by the CAR, which limits the TCR’s function and allows tumor cells lacking BCMA to survive. This reveals a caveat in using dual TCR-CAR strategies for heterogeneous tumors, highlighting the complexity of antigen escape and immune targeting in MM. | | cancer-immunotherapy | chimeric-antigen-receptor-car-t-cell-therapy |
| Chiu, C.-L., Zhang, D., Zhao, H., Wei, Y., Polasko, A. L., Thomsen, M. T., Yang, V., Yang, K. K., Hauck, S., Peterson, E. E., Wen, R. M., Qiu, Z., Corey, E., Miao, Y. R., Rankin, E. B., Peehl, D. M., Huang, J., Giaccia, A. J., & Brooks, J. D. (2024). Targeting AXL inhibits the growth and metastasis of prostate cancer in bone. Clinical Cancer Research, 30(5) | James D. Brooks, Department of Urology, Stanford University School of Medicine, Stanford, CA 94305 | Cancer Biology | Metastasis | BLI | Mouse | Lago | 2025 | This study investigates the role of AXL, a receptor tyrosine kinase, in bone metastases of castration-resistant and neuroendocrine prostate cancer, which are typically lethal after failure of hormonal therapies. The researchers found that activated AXL is highly expressed in prostate cancer bone metastases and is associated with poorer survival. They tested batiraxcept (sAXL), a novel soluble AXL inhibitor, in patient-derived xenograft models implanted in mouse bones. Batiraxcept, alone or combined with chemotherapy drugs docetaxel or carboplatin, significantly inhibited tumor growth and lung metastases. This effect involved downregulating cancer stemness genes and key signaling pathways such as PI3K, JAK, MAPK, and E2F1/NUSAP1. The results suggest batiraxcept is a promising therapeutic option for advanced metastatic prostate cancer. | | cancer-biology | metastasis |
| Sanlorenzo, M., Novoszel, P., Vujic, I., Gastaldi, T., Hammer, M., Fari, O., De Sa Fernandes, C., Landau, A.D., Göcen-Oguz, B.V., Holcmann, M., Monshi, B., Rappersberger, K., Csiszar, A., & Sibilia, M. (2024). Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Nature Cancer. | Maria Sibilia, Center for Cancer Research, Medical University of Vienna, Comprehensive Cancer Center, Vienna, Austria | Cancer Immunotherapy | Tumor Immunology and Metastasis | BLI | Mouse | Lago X | 2025 | This study reveals how combining systemic type I interferon (IFN-I) with topical application of the TLR7/8 agonist imiquimod (IMQ) elicits a potent anti-tumor immune response. Systemic IMQ activates plasmacytoid dendritic cells (pDCs) to produce IFN-I, which primes conventional dendritic cells and macrophages to express TLR7/8. Subsequent topical IMQ treatment activates these primed immune cells via c-Jun/AP-1 signaling to produce IL-12, which suppresses angiogenesis and promotes tumor necrosis. This combined therapy induces robust, CD8⁺ T cell-mediated systemic immunity that inhibits local tumors and distant metastases and enhances the effectiveness of PD-1 immune checkpoint blockade. The findings support translational potential for treating accessible tumors like melanoma or breast cancer. | | cancer-immunotherapy | tumor-immunology-and-metastasis |
| Scholten, D., El-Shennawy, L., Jia, Y., Zhang, Y., Hyun, E., Reduzzi, C., Hoffmann, A. D., Almubarak, H. F., Tong, F., Dashzeveg, N., Sun, Y., Squires, J. R., Lu, J., Platanias, L. C., Wasserfall, C. H., Gradishar, W. J., Cristofanilli, M., Fang, D., & Liu, H. (2025). Rare subset of T cells form heterotypic clusters with circulating tumor cells to foster cancer metastasis. bioRxiv. | Huiping Liu, Northwestern University, Chicago, IL USA | Cancer Biology | Metastasis | BLI | Mouse | Lago | 2025 | This study identifies a rare subset of T cells CD4⁺CD8⁺ double-positive T cells (DPT cells) that form physical clusters with circulating tumor cells (CTCs) in the bloodstream of patients with advanced breast cancer. These clusters are particularly common in triple-negative and luminal B subtypes and are associated with worse patient outcomes. DPT cells, which display features of immune exhaustion and suppression, are up to 140 times more enriched in CTC clusters compared to their frequency in the general white blood cell population. The interaction between DPT cells and CTCs is mediated by the integrin VLA4 on T cells and its ligand VCAM1 on tumor cells. Blocking this interaction in preclinical models using anti-VLA4 antibodies reduced metastasis and improved survival. These findings highlight a new mechanism of immune cell–tumor cell cooperation in cancer metastasis and suggest new biomarker and therapeutic opportunities. | | cancer-biology | metastasis |
| Li, W., Shi, J., Lv, Q., Miao, D., Tan, D., Lu, X., Xiong, H., Luo, Q., Xia, Y., Han, Y., Dong, X., Huang, G., Zhang, X., & Yang, H. (2025). Identification of the LCOR-PLCL1 pathway that restrains lipid accumulation and tumor progression in clear cell renal cell carcinoma. International Journal of Biological Sciences, 21(5), 2296–2312. | Hongmei Yang, Department of Pathogenic Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China | Cancer Biology | Renal Cell Carcinoma | FLI | Mouse | Lago | 2025 | This study explores the role of the LCOR-RUNX1-PLCL1 signaling axis in clear cell renal cell carcinoma (ccRCC), a cancer marked by abnormal lipid accumulation. The researchers discovered that LCOR alleviates RUNX1-mediated repression of PLCL1, resulting in increased PLCL1 expression. PLCL1, in turn, reduces lipid buildup through UCP1-driven lipid browning and promotes tumor cell apoptosis via activation of p38 MAPK signaling. These findings identify LCOR as a potential diagnostic marker and therapeutic target in ccRCC, offering new insight into how lipid metabolism contributes to tumor progression. | | cancer-biology | renal-cell-carcinoma |
| Meany, E. L., Williams, C. M., Song, Y. E., Doulames, V. M., Bailey, S. J., Williams, S. C., Jons, C. K., Fox, P. M., & Appel, E. A. (2025). Preventing peritendinous adhesions using lubricious supramolecular hydrogels. bioRxiv. | Eric A. Appel, Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA | Biomedical Engineering | Anti-Adhesion Biomaterials | BLI | Rat | Lago X | 2025 | This study tackles the common complication of peritendinous adhesions, which form in a significant portion of patients after flexor tendon injuries and can severely limit range of motion. The researchers developed dynamically crosslinked, bioresorbable supramolecular hydrogels that serve as lubricious, injectable barriers to prevent adhesion formation. These hydrogels are easy to store and apply, maintain their structure during the critical early stages of healing, and do not impair tendon movement or strength. Tested in cadaveric human hands and a preclinical rat model, the hydrogels remained at the application site, supported tendon healing, and improved functional recovery, showing strong potential for clinical use. | | biomedical-engineering | anti-adhesion-biomaterials |
| Meng, Z., Tang, M., Xu, S., Zhou, X., Zhang, Z., Yang, L., Nüssler, A. K., Liu, L., & Yang, W. (2025). Protective effects of bone marrow mesenchymal stem cell-derived exosomes loaded cerium dioxide nanoparticle against deoxynivalenol-induced liver damage. Journal of Nanobiotechnology, 23, Article 215. | Wei Yang, Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan, 430030, China | Nanomedicine | Exosome-based Therapy | BLI | Mouse | Lago X | 2025 | This study developed a novel therapeutic strategy using bone marrow mesenchymal stem cell-derived exosomes (BMSC-exos) loaded with cerium dioxide nanoparticles (CeO₂ NPs) to counteract liver damage caused by the mycotoxin deoxynivalenol (DON). The hybrid system (BMSC-exos@CeO₂) exhibited low toxicity, enhanced antioxidant activity, and reduced inflammation in vitro and in vivo. Multi-omics analysis revealed its impact on metabolic and signaling pathways, including AMPK and JAK1/STAT3, indicating its effectiveness in protecting the liver from oxidative and inflammatory stress. | | nanomedicine | exosome-based-therapy |
| Wang, Y., Wu, J., Hartzell, E. J., Hu, W., Mahle, R., Li, X., Chen, Y., Sahoo, J. K., Chan, C., Longo, B. N., Jacobus, C. S., Li, C., & Kaplan, D. L. (2025). Living plastics from plasticizer-assisted thermal molding of silk protein. Nature Communications, 16, Article 52. | David L. Kaplan, Department of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA | Biomaterials | Microbial Encapsulation | FLI | Mouse | Lago X | 2025 | This study presents a sustainable method for producing "living plastics" by incorporating active microorganisms into silk protein matrices using a low-temperature, plasticizer-assisted thermal molding process. The resulting silk-based plastics provide mechanical robustness while preserving microbial viability. Encapsulated probiotics survive gastrointestinal conditions more effectively, and embedded soil rhizobacteria maintain protease activity to accelerate biodegradation in soil. This work highlights the dual functionality of silk-based living plastics: enhanced biological performance and environmental sustainability. | | biomaterials | microbial-encapsulation |
| Gebeyehu, E. K., Shresth, R., Saha, T., Tienaho, J., Jauhiainen, U., Tarhini, A. A., & Tehrani‑Bagha, A. R. (2025). Antibacterial and physicomechanical properties of cellulosic nonwovens functionalized with chitosan: A study on interaction effects of influencing factors and assessment methods. Bioresources and Bioprocessing, 12(11). | Ali Amin Tarhini, Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Vuorimiehentie 1, 02150, Espoo, Finland | Materials Science | Antimicrobial Textiles | BLI | Bacteria | Lago X | 2025 | This study investigates the antibacterial and mechanical performance of cellulosic nonwoven fabrics functionalized with chitosan, a natural biopolymer. The researchers systematically evaluated the effects of chitosan’s molecular weight (low, medium, high), concentration (1–15 g/L), and application method (pad-dry vs. dip-dry) on bacterial inhibition and fabric properties. Using three antibacterial assessment methods, they found that dip-dry application of low-molecular-weight chitosan at ≥5 g/L showed the most effective E. coli inhibition (complete bacterial reduction), as confirmed by colony-forming unit counts. The study also highlights the synergistic enhancement of antibacterial effects when chitosan is applied to cellulose rather than tested in solution. Additionally, treatment altered fabric properties like tensile strength and stiffness. These findings support optimized chitosan use in eco-friendly, high-performance antimicrobial textiles. | | materials-science | antimicrobial-textiles |
| Wu, Y., Wang, P., Liu, L. X., Gao, C., Qin, Q., Hageman, M., Kirkland, T. A., Su, Y., & Lin, M. Z. (2025). Rapid optimization of protein function in mammalian cells via microbe-independent deep assembly and screening. bioRxiv. | Michael Z. Lin, Department of Bioengineering, Stanford University, Stanford, CA, USA | Neurobiology | Biosensors | BLI | Mouse | Lago X | 2025 | This study presents MIDAS (Microbe-Independent Deep Assembly and Screening), a new technique for rapidly optimizing protein function directly in mammalian cells, bypassing traditional microbial cloning steps. MIDAS combines PCR-based library construction, direct transfection of linear DNA into cells, and phenotypic screening, allowing for efficient evaluation of all variants at specific mutational sites. The authors applied this method to develop the first bioluminescent acetylcholine indicator (ACh-NeuBI). Using five rounds of optimization, they improved its responsivity by 29-fold, enabling noninvasive, real-time detection of acetylcholine in vivo with responses up to 100-fold. | | neurobiology | biosensors |
| Coates, I. A., Driskill, M. M., Rajesh, N. U., Lipkowitz, G., Ilyin, D., Xu, Y., Dulay, M. T., Jacobson, G. B., Perry, J. L., Tian, S., & DeSimone, J. M. (2025). Free-form microfluidic microneedle array patches. bioRxiv. | Joseph M. DeSimone, 1Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA | Biomedical Engineering | Drug Delivery | BLI/FLI | Mouse | Lago X | 2025 | This study introduces a novel class of microfluidic microneedle array patches (MAPs) designed for personalized, transdermal drug delivery. By integrating microfluidic channels with microneedle structures and using a high-resolution additive manufacturing method called injection continuous liquid interface production (iCLIP), the researchers created MAPs capable of delivering a wide variety of payloads—including liquids and reconstituted solid-state drugs—at tunable volumes. Inspired by biological systems like stingers and fangs, the microneedles are engineered to resist clogging, enhance strength, and prevent leakage. The platform supports multi-payload delivery, combinational therapies, and point-of-care reconstitution, offering a scalable and highly versatile solution for next-generation drug administration. | | biomedical-engineering | drug-delivery |
| Tang, M., Mahri, S., Shiau, YP. et al. Multifunctional and Scalable Nanoparticles for Bimodal Image-Guided Phototherapy in Bladder Cancer Treatment. Nano-Micro Lett. 17, 222 (2025). | Tzu-Yin Lin, Division of Hematology/Oncology, Department of Internal Medicine, University of California Davis, Sacramento, CA 95817, USA | Cancer | Bladder Cancer | BLI | Mouse | Lago X | 2025 | This study presents a multifunctional nanoparticle platform—pyropheophorbide a–bisaminoquinoline conjugate lipid nanoparticles (PPBC LNPs)—designed for both bladder cancer treatment and real-time imaging. These nanoparticles combine photodynamic therapy (PDT), photothermal therapy (PTT), and autophagy inhibition to effectively kill cancer cells. They also offer dual-mode imaging through photoacoustic (PA) and fluorescence (FL) modalities, enabling accurate tracking of drug distribution and predicting treatment outcomes. Formulated with lipid-based materials via microfluidics, the PPBC LNPs demonstrate strong tumor accumulation, long tumor retention, and significant therapeutic effects, including complete tumor ablation in some cases—highlighting their promise for clinical application. | | cancer | bladder-cancer |
| Mawaki, A., Kohta, M., Yoshimura, A., Nakatani, T., Nagao, S., & Sugama, J. (2024). Effect of docetaxel administration on fluid dynamics in mice. Japan Journal of Nursing Science, 21(1). | Junko Sugama, PhD, RN Research Center for Implementation Nursing Science Initiative, Fujita Health University, 1-98, Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan | Cancer | Edema | FLI | Mouse | Lago X | 2025 | This study investigated how the chemotherapeutic drug docetaxel affects fluid dynamics in mice. Mice treated with docetaxel showed increased leakage of blood plasma into tissues, an early sign of edema, though there were no major changes in interstitial fluid volume or lymphatic transport. The findings suggest this mouse model may be useful for studying edema development and testing preventive strategies in cancer therapy. | | cancer | edema |
| Xue Z, Qin L, Xuan H, Luo K, Huang M, Xie L, Su Y, Xu L, Harsh J, Dale B, Shi X, Chen X, Kaniskan HÜ, Jin J, Wen H. (2024) A potent and selective ENL degrader suppresses oncogenic gene expression and leukemia progression. Sci Adv. | Hong Wen, Department of Epigenetics, Van Andel Institute, Grand Rapids, MI, USA | Cancer | Leukemia | BLI | Mouse | Ami HTX | 2024 | The paper presents the development of MS41, a selective degrader of the ENL protein, designed to inhibit leukemia progression, particularly in mixed-lineage leukemia-rearranged (MLL-r) leukemia. MS41 recruits the von Hippel–Lindau (VHL) E3 ubiquitin ligase to degrade ENL, effectively reducing chromatin occupancy of ENL-associated transcription machinery and suppressing oncogenic gene expression. In vitro, MS41 inhibits leukemia cell proliferation, and in vivo, it significantly delays leukemia progression and improves survival in a mouse model. Bioluminescence imaging (BLI) was used to monitor leukemia progression and the therapeutic effects of MS41, providing real-time visualization of leukemia cell proliferation and dissemination. MS41 selectively degrades ENL without affecting other YEATS domain-containing proteins and shows no detectable in vivo toxicity. The compound has only mild, reversible effects on normal hematopoiesis and is highlighted as a promising therapeutic candidate for ENL-dependent cancers, including MLL-r leukemia and potentially other cancers with ENL YEATS domain mutations. | | cancer | leukemia |
| Zheng, D., Bhuvan, T., Payne, N.L. et al. (2024) Subcutaneous delivery of mesenchymal stromal cells induces immunoregulatory effects in the lymph node prior to their apoptosis. Stem Cell Res Ther | "Tracy S. P. Heng, Department of Anatomy and Developmental Biology, Biomedicine
Discovery Institute, Monash University, Clayton, VIC 3800, Australia" | Cell Therapies | MSCs Therapy | BLI | Mouse | Ami HTX | 2024 | The paper investigates the immunomodulatory effects of mesenchymal stromal cells (MSCs) delivered via subcutaneous (SC) injection into inflamed tissue. A mouse model of localized skin inflammation was used to study the effects of SC-injected MSCs on the local lymph node (LN). The study found that SC injection of MSCs into inflamed skin induced an expansion of IL-10-producing macrophages and activated regulatory T cells (Tregs) in the draining LN. This immunoregulatory effect was not observed with apoptotic MSCs or when MSCs were injected into non-inflamed tissue. The findings suggest that SC-injected MSCs exert anti-inflammatory effects in the draining LN prior to their apoptosis, contrasting with intravenously delivered MSCs where anti-inflammatory effects are linked to their apoptosis. Bioluminescence imaging (BLI) was used to monitor the persistence and localization of MSCs in vivo. | | cell-therapies | mscs-therapy |
| Byrne CM, Márquez AC, Cai B, Coombs D, Gantt S. (2024) Spatial kinetics and immune control of murine cytomegalovirus infection in the salivary glands. PLoS Comput Biol. | "Soren Gantt, Departement de Microbiologie, Infectiologie et Immunologie, Universite de Montreal, Montreal, Quebec, Canada" | Immunology | Viral Infections | BLI | Mouse | Ami HTX | 2024 | The paper investigates the relationship between immune response and viral replication in the salivary glands (SG) of mice infected with murine cytomegalovirus (MCMV). Mice were infected with low doses of MCMV, and viral and immunological dynamics were characterized in the SG, blood, and spleen. Viral loads expand faster and decay slower in the SG compared to other organs, with IE1-specific CD8 T cells and KLRG1+ NK cells playing significant roles in controlling infection. CD4 T cell-mediated cytokine release, particularly IFN-γ and TNF-α, is critical for inhibiting MCMV replication in the SG. Mathematical models predict a high within-host basic reproductive number (R0) for MCMV in the SG and highlight the importance of organ-specific immune responses. Bioluminescence imaging (BLI) was used to monitor viral spread and immune response dynamics in mice. | | immunology | viral-infections |
| Preprint: James A C Bertlin, Tekle Pauzaite, Qian Liang, Niek Wit, James C Williamson, Jia Jhing Sia, Nicholas J Matheson, Brian M Ortmann, Thomas J Mitchell, Anneliese O Speak, Qing Zhang, James A Nathan (2024) VHL synthetic lethality screens uncover CBF-β as a negative regulator of STING, bioRxiv | "James A Nathan, Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, Department of Medicine, University of Cambridge, Cambridge, CB2 0AW, UK" | Cancer | Renal cancer | BLI | Mouse | Ami HTX | 2024 | The paper investigates synthetic lethal interactions in clear cell renal cell carcinoma (ccRCC) with VHL gene inactivation, identifying Core Binding Factor β (CBF-β) as a key player. Genome-wide CRISPR/Cas9 screening revealed that loss of CBF-β leads to cell death in VHL-null ccRCC cells and impairs tumor growth in vivo. This synthetic lethality is independent of hypoxia-inducible factors (HIFs) but involves the derepression of STING, which upregulates type I interferon signaling. Targeting CBF-β selectively induces tumor cell lethality and enhances type I interferon signaling, offering a potential therapeutic strategy for ccRCC. Bioluminescence imaging (BLI) was used in xenograft experiments to monitor tumor growth and metastasis in mice, confirming the relevance of CBF-β/VHL synthetic lethality in vivo. Overall, the study highlights CBF-β as a promising target for ccRCC treatment. | | cancer | renal-cancer |
| Zhu, Y., Liu, X., Chen, X. et al. (2024) Adipose-derived stem cells apoptosis rejuvenate radiation-impaired skin in mice via remodeling and rearranging dermal collagens matrix. Stem Cell Res Ther | "Xihang Chen, Yunjun Liao, Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, 1838 Guangzhou North Road, Guangzhou 510515, Guangdong, People’s Republic of China" | Stem Cells | Cell-based Therapies | FLI | Mouse | Ami HTX | 2024 | The study investigates the therapeutic potential of adipose-derived stem cells (ADSCs) for treating chronic radiation dermatitis (CRD), a common side effect of radiotherapy. ADSCs were found to effectively mitigate CRD symptoms, including acanthosis, fibrosis, and irregular collagen deposition. Transcriptome sequencing and histologic analysis suggested that ADSCs promote dermal extracellular matrix remodeling. Fluorescence lifetime imaging (FLI) was used to track the fate of transplanted ADSCs, revealing extensive apoptosis within a few days. Surprisingly, this apoptosis was essential for their therapeutic efficacy, as inhibiting apoptosis reduced their beneficial effects. Mechanistically, ADSCs were shown to remodel collagen fibers, counteracting the atrophy and rupture caused by irradiation. | | stem-cells | cell-based-therapies |
| Radhakrishnan H, Newmyer SL, Ssemadaali MA, Javitz HS, Bhatnagar P. (2024) Primary T-cell-based delivery platform for in vivo synthesis of engineered proteins. Bioeng Transl Med. | "Eun Ji Chung, a Parijat Bhatnagar, Biosciences Division, SRI International, Menlo Park, CA 94025, USA. | Immunology | Cell-based Therapies | BLI | Mouse | Ami HTX | 2024 | The paper presents a novel cell-based delivery platform using primary T cells engineered to synthesize therapeutic proteins directly at the disease site, minimizing systemic toxicity. The platform leverages T cells' ability to migrate to disease sites and activate in response to specific antigens, ensuring precise and localized drug delivery. The study optimized various parameters to enhance the transduction and expansion of engineered T cells, achieving significant improvements in production yield. The engineered T cells were validated in vivo, demonstrating their ability to synthesize functional proteins proportionate to the disease burden. Bioluminescence imaging (BLI) was used to monitor the delivery of therapeutic proteins, confirming the platform's efficacy in targeting tumor cells and reducing its burden. This innovative approach offers a promising solution for treating various diseases by providing a controlled and targeted delivery of biologics, potentially transforming the landscape of cell-based therapies. | | immunology | cell-based-therapies |
| Yi Huang, Jonathan Wang, Valeria Mancino, Jessica Pham, Colette O’Grady, Hui Li, Kairui Jiang, Deborah Chin, Christopher Poon, Pei-Yin Ho, Georgina Gyarmati, János Peti-Peterdi, Kenneth R Hallows, Eun Ji Chung (2024) Oral delivery of nanomedicine for genetic kidney disease, PNAS Nexus | "Eun Ji Chung, a
Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA" | Genetics | Kidney Disease | FLI | Pig & Mouse | Ami HTX | 2024 | The paper discusses the development of a novel nanomedicine strategy for treating autosomal dominant polycystic kidney disease (ADPKD) using chitosan particles (CP) for oral delivery of kidney-targeting peptide amphiphile micelles (KMs). The encapsulation of KMs into CP enhances the bioavailability and absorption of the therapeutic peptides in the intestines, leading to greater accumulation in the kidneys compared to intravenous administration. The study demonstrates that KM-CP loaded with the ADPKD drug metformin (KM-CP-met) significantly improves therapeutic efficacy in ADPKD mice without adverse side effects. Fluorescence lifetime imaging (FLI) was used to evaluate the mechanism of KM access to the kidneys, showing that KMs pass through the glomerular filtration barrier and accumulate in the renal tubular system. The results indicate that KM-CP-met offers a promising approach for the long-term treatment of chronic kidney diseases by improving drug delivery and minimizing off-target effects. This innovative strategy could potentially transform the management of genetic kidney diseases by providing a practical and effective oral treatment option. | | genetics | kidney-disease |
| Casanova, A.G., Roth, G.S., Hausmann, S. et al. (2024) Cytoskeleton remodeling induced by SMYD2 methyltransferase drives breast cancer metastasis. Cell Discovery | Pawel K. Mazur or Nicolas Reynoird: Grenoble Alpes University, CNRS UMR 5309, INSERM U 1209, Institute for Advanced Biosciences,or Grenoble, France 2 Clinique Universitaire d’Hépato-gastroentérologie et Oncologie digestive, CHU Grenoble Alpes, Grenoble, France | Cancer | Breast Cancer | BLI | Mouse | Ami HTX | 2024 | The article investigates the role of SMYD2 in tripple negative breast cancer metastasis, showing it methylates BCAR3 at lysine 334, which recruits FMNLs to enhance cell motility. Inhibition of SMYD2 reduces metastasis in mouse models and patient-derived xenografts. Bioluminescence imaging (BLI) was used to monitor metastatic spread, demonstrating that cells proficient in SMYD2-BCAR3 signaling formed multiple tumor foci, while those with impaired methylation showed significantly lower BLI signals. Targeting the SMYD2-BCAR3-FMNL axis could be a promising therapeutic strategy to prevent breast cancer metastasis. | | cancer | breast-cancer |
| Cheng Zhang et al. (2024) Von Hippel Lindau tumor suppressor controls m6A-dependent gene expression in renal tumorigenesis, The Journal of Clinical Investigation | Qing Zhang, UT Southwestern Medical Center, 5323 Harry Hines Blvd., NB7.208, Dallas, Texas 75390-9072, USA | Cancer | Renal cancer | BLI | Mouse | Ami HTX | 2024 | The article discusses the role of the Von Hippel Lindau (VHL) tumor suppressor gene in regulating m6A RNA modification in clear cell renal cell carcinoma (ccRCC). VHL interacts with m6A writer proteins METTL3 and METTL14, promoting m6A modification, which stabilizes PIK3R3 mRNA and suppresses PI3K/AKT signaling, thereby inhibiting tumor growth. The study found that VHL depletion leads to decreased m6A levels and faster decay of PIK3R3 mRNA, resulting in increased PI3K/AKT activity and tumor progression. Bioluminescence imaging (BLI) was used to monitor tumor growth in vivo, showing that PIK3R3 depletion led to increased tumor progression and metastasis, while PIK3R3 overexpression reduced tumor growth. The findings suggest that targeting the VHL/m6A/PIK3R3 axis could be a potential therapeutic strategy for ccRCC. Overall, the study highlights the importance of VHL in regulating mRNA stability and tumor suppression through m6A modification. | | cancer | renal-cancer |
| Jialu Zhang, Zhengyuan Wang, Dingyi Zhang, Qiyan Chen, Jiawei Xu, Luxia Tang, Jinyan Luo, Qiusui Mai, Xia Lu, Leyi Tan, Ning Gan, Qianli Jiang (2024) Development of a precision tumor bone metastasis model by a magnetic micro-living-motor system, Colloids and Surfaces B: Biointerfaces | Qianli Jiang, Department of Haematology, Nanfang Hospital, Southern Medical University, Guangzhou, China.** Department of Biotechnology, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan." | Cancer | Bone cancer | BLI/X-ray | Mouse | Ami HTX | 2024 | The paper presents a novel method for modeling bone metastasis using a magnetic micro-living-motor (MLM) system. This system combines Fe₃O₄-PDA-Au nanoparticles with tumor cells to achieve precise migration and colonization in bone under a magnetic field. Bioluminescence imaging (BLI) was used to track tumor growth, showing that 82.76% of mice in the MLM group developed bone metastasis by day 3, compared to 56.82% in the caudal artery (CA) injection group. X-ray imaging confirmed successful intra-bone marrow injections and precise tumor localization. The MLM system significantly reduced metastasis to vital organs by approximately 90% compared to the CA group. This innovative approach enhances the consistency and reliability of bone metastasis models, offering a valuable tool for studying tumor mechanisms and testing new treatments. | | cancer | bone-cancer |
| Atsushi Minami, Takehiro Asai, Tsuyoshi Tachibana, Yu Tanaka, Mitsuyuki Nakajima, Shiori Tamura, Morihiro Nakazawa, Yoshiharu Tsuru, Yoichi Fujiyama, Yoh-ichi Tagawa, Tomohisa Kuzuyama, Shigeru Kakuta, Tetsuhiro Ogawa (2024) Establishment of the improved colonization of Escherichia coli laboratory strain in the intestine mediated by single gene deletion,Biochemical and Biophysical Research Communications, | "Shigeru Kakuta*, Tetsuhiro Ogawa** * Department of Veterinary Medical Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
** Department of Biotechnology, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan." | Infection | E. Coli | BLI | Mouse | Ami HTX | 2024 | The paper investigates enhancing the colonization efficiency of Escherichia coli in the intestine by deleting the RNase I gene. This deletion improves biofilm formation, leading to better stability and colonization in the intestinal tract. The Δrna strain shows superior colonization in both in vitro and in vivo experiments, confirmed by fluorescence in situ hybridization (FISH) and competitive assays. Bioluminescence imaging (BLI) visualizes and quantifies bacterial colonization. The study proposes a technique to improve probiotic therapies by manipulating a single gene, contributing to advancements in gut microbiome research. | | infection | e-coli |
| Preprint: Alain Valdivia, Adebimpe Adefolaju, Morrent Thang, Luz Andrea Cuaboy, Catherine John, Breanna Mann, Andrew Satterlee, Victoria L Bae-Jump, Shawn Hingtgen (2024) A Novel ex-vivo platform for personalized treatment in metastatic ovarian cancer, bioRxiv | Shawn Hingtgen, 4212 Marsico Hall, 125 Mason Farm Road, Chapel Hill, NC 27599, USA. | Cancer | ovarian cancer | BLI | Rat | Ami HTX | 2024 | The paper introduces the Organotypic Mesentery Membrane Culture (OMMC) model for personalized treatment of metastatic ovarian cancer. This ex-vivo platform uses living rat mesenteric membranes to rapidly engraft and assess human ovarian cancer development and progression. Bioluminescence imaging (BLI) was employed to track tumor growth and response to treatments, demonstrating consistent viability and interaction with the mesentery. The study calculated drug sensitivity scores (DSS) to predict therapeutic outcomes, showing positive correlations with clinical responses. | | cancer | ovarian-cancer |
| Preprint: Amanda N. Pope, Devon L. Moose, Guy O. Hudson, Hank R. Weresh, Eric B. Taylor, Michael D. Henry (2025) Brief Pulses of High-Level Fluid Shear Stress Enhance Metastatic Potential and Rapidly Alter the Metabolism of Cancer Cells, bioRxiv | "Michael D. Henry, Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, IA 52242" | Cancer | prostate and breast cancer | BLI | Rat | Ami HTX | 2025 | The study investigates how brief pulses of high-level fluid shear stress (FSS) affect cancer cells' metastatic potential and metabolism. Cancer cells exposed to FSS showed increased invasiveness and anchorage-independent proliferation in vitro, and enhanced metastatic colonization in vivo. In vivo bioluminescence imaging (BLI) was used to track the development of metastatic colonies, showing that FSS-exposed cells formed metastases more rapidly. FSS exposure rapidly altered the cells' metabolism, promoting survival by providing energy for cytoskeletal remodeling and reducing oxidative stress. These findings suggest that FSS exposure enhances cancer cells' ability to survive and proliferate during metastasis, highlighting potential therapeutic targets to reduce metastatic colonization. | | cancer | prostate-and-breast-cancer |
| Filipiuc, S.I. Simionescu, N. Stanciu, G.D. Coroaba, A. Marangoci, N.L.Filipiuc, L.E. Pinteala, M. Uritu, C.M. Tamba, B.I. (2025) Fluorescent Rhein-Loaded Liposomes for In Vivo Biodistribution Study. Pharmaceutics | Cristina Mariana Uritu, Centre of Advanced Research in Bionanoconjugates and Biopolymers, “Petru Poni” Institute of Macromolecular Chemistry, 700487 Iasi, Romania | Regenerative Medicine | liposomes | FLI | Rat | Ami HTX | 2025 | The study developed and investigated Rhein-loaded liposomes (Lip-Rh) to enhance Rhein's water solubility and oral bioavailability. The liposomes were characterized and tested for cytotoxicity on tumor and normal cells. In vivo fluorescence imaging (FLI) showed that Lip-Rh had significantly improved oral bioavailability, with rapid absorption into the bloodstream. FLI also helped visualize the biodistribution of Lip-Rh, confirming its targeted delivery to metabolic organs. The results suggest that Lip-Rh is a promising candidate for oncological treatments due to its increased cytotoxicity towards tumor cells and protection of normal tissues. Further research is needed to explore its potential in anticancer therapy | | regenerative-medicine | liposomes |
| Yang, X., Ma, X., Zhao, T. et al. (2025) Activation of CAMK2 by pseudokinase PEAK1 represents a targetable pathway in triple negative breast cancer. Nat Commun | Roger J. Daly, Cancer Program, Monash Biomedicine Discovery Institute, Clayton, VIC, Australia. 2 Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC, Australia | Cancer | Breast Cancer | BLI | Mouse | Ami HTX | 2025 | The study focuses on triple-negative breast cancer, particularly on the pseudokinase PEAK1 and its effectors, specifically calcium/calmodulin-dependent protein kinase 2 (CAMK2D). Researchers tested inhibitors for CAMK2D and confirmed that these inhibitors reduce PEAK1-dependent migration in vitro. In vivo testing demonstrated that these inhibitors reduce metastatic potential, as shown through BLI imaging. | | cancer | breast-cancer |
| Preprint: Luo, J. et al. (2025), Harnessing Telodendrimer Nanotraps in Nanogels for Systemic Immune Modulation in Sepsis Treatment. | "Juntao Luo, State University of New York (SUNY) - SUNY Upstate Medical University
College of Health Professions, Department of Physical Therapy. Syracuse, NY 13210 United States" | Immunology | sepsis | BLI | Mouse | Ami HTX | 2025 | The study focuses on a novel sepsis treatment. Scientists have developed nano-sized hydrogels designed to scavenge septic molecules. In vivo imaging was utilized to evaluate the biodistribution of these nano-sized TG hydrogel particles and to assess their efficacy in treating LPS-induced inflammation and CLP-induced sepsis. | | immunology | sepsis |
| Hu, X., Tediashvili, G., Gravina, A. et al. (2025) Inhibition of polymorphonuclear cells averts cytotoxicity against hypoimmune cells in xenotransplantation. Nat Commun 16, 3706. | Sonja Schrepfer, Sana Biotechnology Inc., 1 Tower Place, South San Francisco, CA 94080, USA | Regenerative Medicine | Immune-evasive cell engineering | BLI | Mouse | Lago | 2025 | This study advances the development of hypoimmune (HIP) cell therapies for xenotransplantation by engineering immune-evasive endothelial cells (ECs) compatible with both non-human primate (NHP) and human immune systems. While HIP ECs are protected from most adaptive and innate immune responses, the researchers identified that polymorphonuclear cells (PMNs) still mediate xenogeneic cytotoxicity. By using a multi-drug regimen or genetically overexpressing PMN-inhibitory ligands (CD99 and CD200), the team was able to prevent PMN-mediated killing. These findings enhance the potential for allogeneic and xenogeneic cell therapies in clinical applications without immunosuppression. | | regenerative-medicine | immune-evasive-cell-engineering |
| Barra, J. M., Robino, R. A., Castro-Gutierrez, R., Proia, J., Russ, H. A., & Ferreira, L. M. R. (2024). Combinatorial genetic engineering strategy for immune protection of stem cell-derived beta cells by chimeric antigen receptor regulatory T cells. Cell Reports, 43(11), 114994. | Holger Russ, Diabetes Institute, University of Florida, Gainesville, FL 32610, USA | Regenerative Medicine | Stem Cell Therapy | BLI | Mouse | Ami HT | 2024 | This study presents a novel strategy to protect stem cell-derived beta cells (sBCs) from immune rejection in the context of type 1 diabetes using engineered regulatory T cells (CAR-Tregs). The authors genetically modify human pluripotent stem cells (hPSCs) to express a biologically inert, truncated version of the epidermal growth factor receptor (EGFRt), which serves as a synthetic and selective target for chimeric antigen receptor (CAR)-Tregs. These EGFR-specific CAR-Tregs are shown to home to and suppress immune responses around EGFRt-expressing sBCs in vitro and in vivo, without systemic immunosuppression. This proof-of-concept demonstrates that co-engineering both graft cells and immune cells offers a targeted, modular, and generalizable immune protection strategy for advancing regenerative medicine therapies. | | regenerative-medicine | stem-cell-therapy |
| Andolino, C., Cotul, E. K., Xianyu, Z., Li, Y., Bhat, D., Ayers, M., Buhman, K. K., Hursting, S. D., Wendt, M. K., & Teegarden, D. (2024). Fatty acid synthase-derived lipid stores support breast cancer metastasis. Cancer & Metabolism, 12, 1–15. | Dorothy Teegarden, Purdue University, West Lafayette, IN USA | Cancer | Breast Cancer | BLI | Mouse | Ami HT | 2024 | This study investigates how lipid metabolism, specifically fatty acid (FA) synthesis and storage, contributes to breast cancer metastasis. The researchers compared metastatic and non-metastatic breast cancer cells and found that metastatic cells accumulate more lipids despite similar fatty acid uptake. These cells enhance de novo lipogenesis using glucose and glutamine to produce citrate, a precursor for FA synthesis. Elevated levels of fatty acid synthase (FASN) were found in metastatic cells, and inhibition of FASN impaired cell migration, survival in detachment (anoikis), and metastasis in vivo. The findings suggest that lipid droplets derived from FASN activity serve as energy sources during metastasis and may support cancer cell survival and growth in stressful conditions. Proteomic analysis also showed that inhibiting FASN disrupted key cellular processes, emphasizing its role in disease progression. | | cancer | breast-cancer |
| Vu Ngo, Serene Xavier, Vishal Khairnar et al. CEACAM1 Orchestrates Lipid Raft Dynamics and B-cell Receptor Signaling in Mantle Cell Lymphoma, 01 November 2024, PREPRINT | Markus Müschen, Yale University, New Haven, CT, USA | Cancer | Hematologic Oncology | BLI | Mouse | Lago X | 2024 | This study reveals a previously unrecognized activating role for CEACAM1 in mantle cell lymphoma (MCL), a type of B-cell cancer. Using a genome-wide loss-of-function CRISPR screen, the researchers identified CEACAM1 as essential in a subset of MCL tumors. Mechanistically, CEACAM1 enhances B-cell receptor (BCR) signaling through two key processes: stabilizing lipid rafts via interaction with the actin cytoskeleton through filamin A, and recruiting the kinase SYK to the BCR complex. Although CEACAM1 is typically classified as an inhibitory receptor due to its ITIM motifs, this study shows it can act as a context-dependent activator in MCL. These findings provide new insights into the regulation of BCR signaling and suggest potential therapeutic targets in B-cell malignancies. | | cancer | hematologic-oncology |
| Wang, Y., Wen, Y., Kim, K. et al. Functional outcome of the anterior vaginal wall in a pelvic surgery injury rat model after treatment with stem cell-derived progenitors of smooth muscle cells. Stem Cell Res Ther 15, 291 (2024). | Bertha Chen, Department of Obstetrics and Gynecology, Stanford University, Stanford, USA | Regenerative Medicine | Stem Cell Therapy | BLI | Rat | Lago X | 2024 | This study investigates the therapeutic potential of human induced pluripotent stem cell (iPSC)-derived progenitor smooth muscle cells (pSMCs) to restore vaginal function after injury. Using a rat model that simulates vaginal prolapse surgery and menopause, the researchers found that pSMC transplantation improved vaginal smooth muscle contractility and altered extracellular matrix protein expression (such as collagen and elastin) in the vagina and urethra. These results suggest that pSMCs could offer a regenerative treatment option for women experiencing recurrent vaginal prolapse after surgery. | | regenerative-medicine | stem-cell-therapy |
| Herndon, M.E., Ayers, M., Gibson-Corley, K.N., Wendt, M.K., Wallrath, L.L., Henry, M.D., & Stipp, C.S. (2024). The highly metastatic 4T1 breast carcinoma model possesses features of a hybrid epithelial/mesenchymal phenotype. Disease Models & Mechanisms, 17(9). | Christopher Stipp, Department of Biology, University of Iowa, Iowa City, IA 52245, USA | Cancer | Metastasis | BLI | Mouse | Ami HT | 2024 | This study investigates the role of E-cadherin in metastasis using the 4T1 murine breast cancer model, which is highly metastatic yet retains E-cadherin expression. The researchers show that 4T1 cells exhibit a hybrid epithelial/mesenchymal (E/M) phenotype, expressing both epithelial and mesenchymal markers, and can invade collectively without downregulating E-cadherin. Comparatively, related but less metastatic cell lines (4T07, 168FARN, 67NR) display increasingly mesenchymal characteristics in vitro, but these do not correlate with higher metastatic potential in vivo. Manipulating E-cadherin levels did not alter metastasis, suggesting that E-cadherin alone is not a simple metastasis suppressor or promoter. Instead, the study highlights the complexity of epithelial-mesenchymal plasticity in metastasis and positions 4T1 cells as a valuable model for studying hybrid E/M states in cancer progression. | | cancer | metastasis |
| Baker, F. L., Smith, K. A., Mylabathula, P. L., Zúñiga, T. M., Diak, D. M., Batatinha, H., Niemiro, G. M., Seckeler, M. D., Pedlar, C. R., O’Connor, D. P., Colombo, J., Katsanis, E., & Simpson, R. J. (2024). Exercise-induced β₂-adrenergic receptor activation enhances the antileukemic activity of expanded γδ T-cells via DNAM-1 upregulation and PVR/Nectin-2 recognition. Cancer Research Communications, 4(5), 1253–1267. | Richard Simpson, University of Arizona, Tucson, AZ 85724. | Cancer | Exercise Immunology | BLI | Mouse | Lago X | 2024 | This study demonstrates that a brief session of graded exercise (20 minutes) mobilizes cytotoxic gamma-delta (γδ) T cells into the bloodstream, priming them for enhanced antitumor function. These exercise-mobilized γδ T cells exhibit transcriptomic and surface markers linked to cytotoxicity, migration, and cytokine signaling. After expansion with zoledronic acid and IL-2, they show superior killing of hematologic cancer cells in vitro and in vivo (in leukemia-bearing mice). The enhanced effect is mediated through β2-adrenergic receptor signaling and depends on the DNAM-1 pathway. These findings provide a mechanistic link between exercise, β2-AR signaling, and the generation of potent γδ T cell products for adoptive immunotherapy against blood cancers. | | cancer | exercise-immunology |
| Akhavan, D., Subham, S., Jeppson, J. D., Aguilar, B., Wong, R. A., Hibbard, J. C., Hui, S., Wong, J. Y. C., Forman, S. J., Alizadeh, D., & Brown, C. E. (2024). Evaluation of the Immunomodulatory Effects of Radiation for Chimeric Antigen Receptor T Cell Therapy in Glioblastoma Multiforme. Cells, 13(13), 1075. | Christine E. Brown, City of Hope National Medical Center, Duarte, CA, USA
| Cancer | Glioblastoma | BLI | Mouse | Lago X | 2024 | This study explores the potential of combining low-dose radiation (stereotactic radiosurgery, or SRS) with Chimeric Antigen Receptor (CAR) T cell therapy as a treatment strategy for Glioblastoma Multiforme (GBM). Radiation is a standard treatment for GBM and has immunomodulatory effects on the tumor microenvironment (TME), but the optimal dose and how it can enhance CAR T cell efficacy had not been well-defined. Using a murine GBM model, the researchers determined that a conditioning dose of 10 Gray (Gy) radiation enhanced both innate and adaptive immune responses in the TME. When combined with CAR T cells targeting the IL13Rα2 antigen, this treatment led to improved antitumor activity and better memory responses, even protecting against tumors lacking IL13Rα2 expression. The study also identified the c-GAS-STING pathway as playing a role in the enhanced tumor protection. These findings support the strategy of using low-dose radiation in combination with CAR T therapy as a promising therapeutic approach for GBM. | | cancer | glioblastoma |
| Guan, X., Fury, H., Issuree, P. D., Atagozli, T., McManimon, E. E., Shao, P., Li, Y., Chimenti, M., Butler, N. S., Kaplan, M. H., Elliott, D. E., Blazar, B. R., & Ince, M. N. (2025). The Impact of Cell-Intrinsic STAT6 Protein on Donor T Cell-Mediated Graft-Versus-Tumor Effect. International Journal of Molecular Sciences, 26(1), 280. | "Nedim Ince, Department of Internal Medicine, Division of Gastroenterology and Hepatology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA" | Immunology | Bone Marrow Transplantation | BLI | Mouse | Ami HT | 2024 | This study investigates the potential for genetic induction of T helper-2 (Th2) signaling in donor T cells to enhance the graft-versus-tumor (GVT) effect in bone marrow transplantation (BMT), while simultaneously suppressing the lethal graft-versus-host disease (GVHD). Researchers used a mouse model with T lymphocytes that overexpress a constitutively active form of the Th2-associated transcription factor STAT6. This approach preserved the GVT response, which helps prevent tumor recurrence after transplantation, by generating robust quantities of both cytotoxic proteins (like granzymes and interferon-γ) and regulatory Th2 cytokines (like IL4 and IL10). Importantly, this treatment did not result in severe GVHD, suggesting that manipulating STAT6 signaling in donor T cells could be a therapeutic strategy to sustain anti-tumor immunity while limiting the adverse effects of GVHD. | | immunology | bone-marrow-transplantation |
| Aniogo, Eric, et al. “Targeting CEA in Metastatic Triple Negative Breast Cancer with Image-Guided Radiation Followed by Fab-Mediated Chimeric Antigen Receptor (CAR) T-Cell Therapy.” Frontiers in Immunology, vol. 15, 20 Dec. 2024 | John Shively, Department of Immunology and Theranostics, City of Hope, Duarte, CA, United States | Cancer Immunotherapy | CAR-T Cell and Image-Guided Radiation Therapy | BLI | Mouse | Lago | 2024 | This study explores the potential of combining Image-Guided Radiation Therapy (IGRT) with anti-CEA scFab CAR-T cell therapy to improve outcomes in metastatic triple-negative breast cancer (TNBC). Researchers engineered two types of CAR-T cells incorporating CD28zeta and 4-1BBzeta signaling domains and found that CD28z-CAR-T cells exhibited superior cytotoxicity and cytokine release in vitro. In vivo, combining IGRT with CAR-T therapy significantly reduced primary tumor growth and successfully eliminated lung metastases—an effect not seen with either treatment alone. These findings suggest that IGRT enhances CAR-T cell infiltration and persistence, offering a promising strategy to improve CAR-T efficacy in solid tumors. | | cancer-immunotherapy | car-t-cell-and-image-guided-radiation-therapy |
| Wang, Y., Wen, Y., Kim, K., Wu, H., Zhang, J., Dobberfuhl, A. D., & Chen, B. (2024). Functional outcome of the anterior vaginal wall in a pelvic surgery injury rat model after treatment with stem cell-derived progenitors of smooth muscle cells. Stem Cell Research & Therapy, 15(1), 291. | Piotr Rychahou, Markey Cancer Center, Lexington, KY, USA | Cancer | Metastatic Pancreatic Neuroendocrine Tumors (pNETs) | BLI | Mouse | Lago | 2024 | This study investigates the potential of targeting ribonucleotide reductase (RNR), specifically its RRM2 subunit, to enhance the effectiveness of radiotherapy for metastatic pancreatic neuroendocrine tumors (pNETs). Using in vitro and in vivo models, the researchers showed that inhibiting RRM2 with the selective agent 3-AP activated DNA damage response pathways, induced cell cycle arrest, and sensitized pNET cells to radiation. In animal models, combining 3-AP with radiotherapy significantly increased tumor apoptosis and reduced lung metastases. These findings support the use of 3-AP as a promising radiosensitizer to improve outcomes in metastatic pNET treatment. | | cancer | metastatic-pancreatic-neuroendocrine-tumors-pnets |
| Lee, D. C., Ta, L., Mukherjee, P., Duraj, T., Domin, M., Greenwood, B., … Seyfried, T. N. (2024). Amino Acid and Glucose Fermentation Maintain ATP Content in Mouse and Human Malignant Glioma Cells. ASN Neuro, 16(1). | Thomas N. Seyfried, Department of Biology, Boston College, Massachusetts, USA | Cancer | Brain Cancer | BLI | Mouse | Ami HT | 2024 | This study used a luciferin-luciferase bioluminescence ATP assay to examine how glucose, glutamine, and oxygen influence energy metabolism in mouse (VM-M3, CT-2A) and human (U-87MG) glioma cells. The results showed that glutamine is essential for maintaining ATP levels and cell viability, even under low oxygen conditions, while glucose alone was insufficient. ATP production persisted in the absence of glucose and hypoxia, suggesting that neither glycolysis nor oxidative phosphorylation (OxPhos) were the primary sources of energy under these conditions. Inhibition of mitochondrial complex IV confirmed that oxygen consumption did not accurately reflect ATP production, and treatment with a glutaminase inhibitor (DON) reduced both ATP content and succinate levels, pointing to mitochondrial substrate-level phosphorylation in the glutaminolysis pathway as a key mechanism. Bioluminescence imaging (BLI) of luciferase-expressing cells demonstrated that glutamine supports short-term viability, but both glucose and glutamine are required for sustained proliferation. Overall, the study proposes a model in which malignant gliomas depend on the aerobic fermentation of both glucose and glutamine to drive tumor growth and maintain ATP, bypassing the need for efficient OxPhos. | | cancer | brain-cancer |
| Rodgers, L. T., Maloney, B. J., Hartz, A. M. S., & Bauer, B. (2025). Fluorescence-Guided Resection of GL261 Red-FLuc and TRP-mCherry-FLuc Mouse Glioblastoma Tumors. Cancers, 17(5), 734. | "Bjorn Bauer, Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY 40536, USA" | Cancer | Glioblastoma | FLI, BLI | Mouse | Lago | 2025 | This study addresses the translational gap in glioblastoma (GBM) research by developing a preclinical model that incorporates fluorescence-guided surgery, a critical component of clinical treatment. Using the FDA-approved imaging agent 5-aminolevulinic acid (5-ALA), researchers performed tumor resections in two mouse GBM models (TRP-mCherry-FLuc and GL261 Red-FLuc), mimicking clinical conditions more accurately than previous models. Bioluminescence and fluorescence imaging were used to guide resection and assess tumor progression, demonstrating that tumor removal significantly extended survival, reduced weight loss, and slowed disease progression in both models. By integrating surgical intervention into preclinical research, this study provides a valuable platform for testing adjuvant therapies and improving the clinical relevance of GBM treatment strategies. | | cancer | glioblastoma |
| Kaczmarek K, Więckiewicz J, Que I, Gałuszka-Bulaga A, Chan A, Siedlar M, Baran J. Human Soluble TRAIL Secreted by Modified Lactococcus lactis Bacteria Promotes Tumor Growth in the Orthotopic Mouse Model of Colorectal Cancer. Arch Immunol Ther Exp (Warsz). 2024 Jan 6;72(1). | Jarek Baran, Department of Clinical Immunology, Institute of Pediatrics, Jagiellonian University Medical College, Kraków, Poland. | Cancer | Colorectal Cancer and Bacterial-Based Therapeutics | BLI | Mouse | Ami HT | 2024 | In this study, researchers evaluated the use of Lactococcus lactis engineered to secrete human soluble TRAIL (hsTRAIL) for localized delivery in an orthotopic colorectal cancer (CRC) model. HCT116 Red-FLuc cells were engrafted into the cecum of SCID mice, and tumor progression was monitored using bioluminescence imaging (BLI) throughout the study. Optical imaging confirmed tumor establishment, growth, and response to treatments across six experimental groups, including L. lactis(hsTRAIL+) alone or combined with metformin (MetF). While L. lactis(hsTRAIL+) successfully delivered biologically active hsTRAIL to the tumor site, BLI revealed that this approach unexpectedly stimulated tumor growth and was associated with M2-macrophage infiltration and crypt shortening. The study highlights both the utility of optical imaging for longitudinal assessment of tumor dynamics and the importance of evaluating immune responses when developing bacterial-based drug delivery systems for CRC therapy. | | cancer | colorectal-cancer-and-bacterial-based-therapeutics |
| Liu, Y., Guerrero, D. Q., Lechuga-Ballesteros, D., Tan, M., Ahmad, F., Aleiwi, B., … So, S. (2024). Lipid-Based Self-Microemulsion of Niclosamide Achieved Enhanced Oral Delivery and Anti-Tumor Efficacy in Orthotopic Patient-Derived Xenograft of Hepatocellular Carcinoma in Mice. International Journal of Nanomedicine, 19, 2639–2653. | Mei-Sze Chua, Department of Surgery, School of Medicine, Stanford University, Stanford, CA, USA | Cancer | Carcinoma | FLI | Mouse | Lago X | 2024 | This study aimed to improve the bioavailability of niclosamide for hepatocellular carcinoma (HCC) treatment by developing an oral self-microemulsifying drug delivery system (Nic-SMEDDS). Compared to standard niclosamide and its ethanolamine salt (NEN), Nic-SMEDDS demonstrated prolonged retention, a 4.1-fold increase in bioavailability, and superior anti-tumor efficacy in an orthotopic patient-derived xenograft (PDX)-HCC mouse model. To track drug distribution and absorption, researchers utilized fluorescence imaging with VivoTag680® dye and the Lago X imaging system. Imaging revealed time-dependent biodistribution of Nic-SMEDDS, highlighting its absorption via the chylomicron pathway. Quantification was performed using Aura software, enabling precise assessment of drug localization. These findings underscore the potential of Nic-SMEDDS as an effective oral therapy for HCC and demonstrate the value of optical imaging techniques in evaluating drug delivery dynamics, pharmacokinetics, and therapeutic impact in preclinical cancer research. | | cancer | carcinoma |
| Rodgers, L. T., Schulz Pauly, J. A., Maloney, B. J., Hartz, A. M. S., & Bauer, B. (2024). Optimization, Characterization, and Comparison of Two Luciferase-Expressing Mouse Glioblastoma Models. Cancers, 16(11), 1997. | "Bjorn Bauer, Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY 40536, USA" | Cancer | Glioblastoma | BLI | Mouse | Lago | 2024 | This study optimizes tumor engraftment in luciferase-expressing glioblastoma (GBM) models, revealing differences between GL261 Red-FLuc (highly mitotic, vascularized) and TRP-mCherry-FLuc (invasive, necrotic). Using bioluminescence imaging (BLI), researchers found GL261 Red-FLuc required immunocompromised mice for consistent engraftment, highlighting immune response effects. AI-based tumor volume quantification improved analysis efficiency. The findings emphasize careful model selection and engraftment considerations for reliable optical imaging in GBM research. | | cancer | glioblastoma |
| Xiao Z, Alam IS, Simonetta F, Chen W, Scheller L, Murty S, Lohmeyer JK, Ramos TL, James ML, Negrin RS, Gambhir SS. ICOS immunoPET enables visualization of activated T cells and early diagnosis of murine acute gastrointestinal GvHD. Blood Adv. 2022 Aug 23;6(16):4782-4792 | Israt S Alam Molecular, Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, Stanford, CA | Molecular Imaging | ImmunoPET for GvHD Diagnosis | BLI | Mouse | Ami HT | 2022 | This study explores a novel approach for early diagnosis of acute graft-versus-host disease (GvHD) following allogeneic hematopoietic cell transplantation (HCT). Since current diagnostic methods rely on late-stage clinical and pathological criteria, the researchers investigate noninvasive positron emission tomography (PET) imaging using an antibody-based tracer (immunoPET). They identify inducible T-cell costimulator (ICOS) as a promising target for detecting activated T cells involved in GvHD. Using a Zirconium-89-deferoxamine-ICOS monoclonal antibody PET tracer, the study demonstrates in vivo visualization of donor T-cell activation in the intestinal tract of a murine GvHD model. Crucially, the tracer does not interfere with GvHD progression or the beneficial graft-versus-tumor (GvT) effect. These findings highlight ICOS immunoPET as a potential strategy for early, noninvasive GvHD diagnosis before clinical symptoms appear. | | molecular-imaging | immunopet-for-gvhd-diagnosis |
| Su Y, Walker JR, Hall MP, Klein MA, Wu X, Encell LP, Casey KM, Liu LX, Hong G, Lin MZ, Kirkland TA. An optimized bioluminescent substrate for non-invasive imaging in the brain. Nat Chem Biol. 2023 Jun;19(6):731-739. doi: 10.1038/s41589-023-01265-x. Epub 2023 Feb 9. | Thomas A Kirkland Promega Biosciences, LLC, San Luis Obispo, CA USA | Neuroimaging | Bioluminescence Imaging in the Brain | BLI | Mouse | Ami HT | 2023 | This study addresses the challenge of bioluminescence imaging (BLI) in the central nervous system, where existing luciferases and substrates have shown limited performance. The researchers introduce cephalofurimazine (CFz), a newly discovered NanoLuc substrate that significantly enhances brain imaging. When paired with Antares luciferase, CFz produces over 20 times more signal from the brain compared to the standard firefly luciferase with d-luciferin. At standard doses, the Antares–CFz system matches the brightness of AkaLuc–AkaLumine/TokeOni, and higher CFz dosing can further triple the signal output. This advancement enables high-sensitivity application of NanoLuc-based indicators in the brain. The study demonstrates the utility of CFz for video-rate imaging in freely moving mice and for non-invasive calcium imaging of sensory-evoked neuronal activity in genetically defined populations. | | neuroimaging | bioluminescence-imaging-in-the-brain |
| Translational regulation of SND1 governs endothelial homeostasis during stress . Han, Z., Tahmasebi, S. et al. Journal of Clin Investigation. 2025;135(3):e168730. | Zhenbo Han Department of Pharmacology & Regenerative Medicine, University of Illinois College of Medicine, Chicago, Illinois, USA | Cardio Oncology | Cardiotoxicity | BLI | Mouse | Lago X | 2025 | In an in vivo renal carcinoma xenograft model, ramipril was shown to protect against sunitinib-induced vascular dysfunction without compromising the drug’s antitumor efficacy. Mice treated with sunitinib alone exhibited significant tumor regression alongside impaired coronary flow reserve (CFR), whereas co-administration of ramipril maintained tumor suppression—as evidenced by bioluminescence imaging, reduced tumor volume and weight, and lower Ki-67 levels—while restoring CFR. Additionally, ramipril reduced sunitinib-induced DNA damage in cardiac endothelial cells, indicating enhanced DNA damage repair, even though it did not reverse sunitinib’s inhibition of SND1. | | cardio-oncology | cardiotoxicity |
| Xia, Z., Jin, Q., Long, Z. et al. Targeting overexpressed antigens in glioblastoma via CAR T cells with computationally designed high-affinity protein binders. Nat. Biomed. Eng (2024). | Longxing Cao - Westlake Disease Modeling Laboratory, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China.
| Glioblastoma | CART cell therapy | BLI | Mouse | Ami HT | 2024 | In this study the authors tested a novel CAR T cell therapy for glioblastoma using de novo-designed binders (DNDBs) that target EGFR, a common glioblastoma marker. Unlike conventional single-chain variable fragments (scFvs), DNDBs are highly stable and maintain specificity, which reduces issues like misfolding and rapid T cell exhaustion. The DNDB CAR T cells demonstrated significantly improved efficacy against glioblastoma cells both in vitro and in vivo. In mouse models, these CAR T cells showed enhanced tumor infiltration and prolonged persistence within the tumor environment, with robust anti-tumor activity against glioblastoma stem cells and patient-derived glioblastoma cells (via tracking bioluminescent signal from tumors in vivo). Additionally, DNDB CAR T cells exhibited higher expression levels on the cell surface and retained functionality over time, addressing major challenges in CAR T therapy for solid tumors. This approach holds promise for improving the stability, specificity, and effectiveness of CAR T therapies in glioblastoma and possibly other solid tumors. Probe: luciferase. | | glioblastoma | cart-cell-therapy |
| Himanshu Rai, Rishabh Singh, Prahalad Bharti et al. Rhodanine composite fluorescence probes to detect amyloid-beta aggregated species in Alzheimer’s disease models, 06 April 2023, PREPRINT (Version 1) | Himanshu Rai, Indian Institute of Technology (Banaras Hindu University)
| Alzheimer's Disease | Cerebral Amyloid-Beta (Aβ) Plaques | FLI | Mouse | Lago X | 2023 | Molecular near-infrared (NIR) imaging is an emerging pre-clinical tool for labeling Alzheimer's disease (AD) biomarkers, particularly cerebral amyloid-beta (Aβ) plaques. This study presents a series of acceptor-π-donor based NIR probes, featuring rhodanine as the acceptor and coumarin or carbostyril as the donor. The most promising probe, 19, demonstrated a strong binding affinity (Kd = 0.143 μM) for Aβ aggregates, minimal nonspecific interaction with BSA, low cytotoxicity, good brain permeability, stable plasma profile, and fluorescence sustainability across various pH levels. Histological fluorescence imaging showed probe 19's high selectivity and affinity for Aβ plaques, confirmed by immunofluorescence and ThT staining, along with a high signal-to-noise ratio. It was also effective in labeling Aβ in the eye imaginal disc of AD Drosophila larvae. These probes, with their versatile structural scaffold, hold promise for evolving as effective NIR imaging probes for detecting AD biomarkers. Probe: NIR | | alzheimers-disease | cerebral-amyloid-beta-a%ce%b2-plaques |
| Gravina, A et al. Synthetic immune checkpoint engagers protect HLA-deficient iPSCs and derivatives from innate immune cell cytotoxicity. Cell Stem Cell. 2023.30, 1538-1548. | Tobias Duese, 1Transplant and Stem Cell Immunobiology (TSI)-Lab, Department of Surgery, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA | Immunology | Immune Checkpoints | BLI | Mouse | Ami HT | 2023 | Present a new class of agonistic immune checkpoint engagers that protect human leukocyte antigen (HLA)-depleted induced pluripotent stem cell-derived endothelial cells (iECs) from innate immune cells. Engagers with agonistic functionality to their inhibitory receptors TIM3 and SIRPa effectively protect engineered iECs from natural killer (NK) cell and macrophage killing. The SIRPa engager can be combined with truncated CD64 to generate fully immune evasive iECs capable of escaping allogeneic cellular and immunoglobulin G (IgG) antibody-mediated rejection. Synthetic immune checkpoint engagers have high target specificity and lack retrograde signaling in the engineered cells. | | immunology | immune-checkpoints |
| Madhurima, G., Sanjeez, D. PRAMEF2-mediated dynamic regulation of YAP signaling promotes tumorigenesis. 2021. Vol 118 No. 40. | Sanjeev Dasa, Molecular Oncology Laboratory, National Institute of Immunology, New Delhi 110067, India | Tumorigenesis | Ubiquitylation | BLI | Mouse | Lago X | 2021 | Delineated PRAMEF2 regulation under low-nutrient conditions. Also showed that it promotes proteasomal degradation of LATS1 kinase of the Hippo/YAP pathway. LATS1 downregulation triggers nuclear accumulation of the transcriptional coactivator YAP, which induces the expression of proliferative and metastatic genes. Thus, PRAMEF2 promotes malignant phenotype in a YAP-dependent manner. Taken together, the findings reveal a pivotal role for PRAMEF2 in determining YAP oncogenic signaling, which has key implications for tumorigenesis. | | tumorigenesis | ubiquitylation |
| Hu, X. et al. Gypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaques | Sonja Schrepfer, Sana Biotechnology Inc., 1 Tower Place, South San Francisco, CA 94080, USA | Immunology | Genetic engineering of allogenic cell therapeutics | BLI | Rhesus Macaques | Lago | 2023 | Engineered rhesus macaque human hypoimmune pluripotent (HIP) cells and transplanted them intramuscularly into four allogeneic rhesus macaques. The HIP cells survived unrestricted for 16 weeks in fully immunocompetent allogeneic recipients and diferentiated into several lineages, whereas allogeneic wild-type cells were vigorously rejected | | immunology | genetic-engineering-of-allogenic-cell-therapeutics |
| Zhang, Q. et al. Nanomicelle-Microsphere Composite as a Drug Carrier to Improve Lung-Targeting Specificity for Lung Cancer. Pharmaceutics. 2022. 14, 510. | Rongfeng Hu, Anhui Province Key Laboratory of Pharmaceutical Preparation Technology and Application, Key Laboratory of Xin’an Medicine, the Ministry of Education, Anhui Province Key Laboratory of Chinese Medicinal Formula, Plant Active Peptide Function Food Innovative Manufacturing Industry Innovation Team, Anhui University of Chinese Medicine, Hefei 230038, China | Cancer, Lung cancer | Nanomicelle-Microsphere Composite as a Drug Carrier | FLI | Mouse | Ami HT | 2022 | Developed a nanomicelle-microsphere composite, in which doxorubicin (DOX) was loaded with spermine (Spm) modified poly (ethylene glycol)-poly(ε-caprolactone) (PEG-PCL) micelles, and then the nanomicelles were noncovalently adsorbed on the surface of poly (lactic-co-glycolic acid) (PLGA) microspheres. An orthotopic lung cancer implantation model based on C57BL/6 mice was established, and in vivo biodistribution studies confirmed that the complex improved the distribution of DOX in the lungs and displayed notable tumor targeting. Probe: DOX-loaded micelles. | | cancer lung-cancer | nanomicelle-microsphere-composite-as-a-drug-carrier |
| Biswajyoti, S. et al. Human cell transformation by combined lineage conversion and oncogene expression. Oncogene. 2021, 40:5533–5547. | Jussi Taipale, 1 Applied Tumor Genomics Research Program, Research Programs Unit, Faculty of Medicine, University of Helsinki, Helsinki, Finland | Cancer | Cancer Genetics | FLI | Mouse | Lago | 2021 | Combination of oncogenes that is characteristic of liver cancer (CTNNB1, TERT, MYC) induces senescence in human fibroblasts and primary hepatocytes. Reprogramming fibroblasts to a liver progenitor fate, induced hepatocytes (iHeps), makes them sensitive to transformation by the same oncogenes. Tumorigenesis is triggered by a combination of three elements: the set of driver mutations, the cellular lineage, and the state of differentiation of the cells along the lineage. Probe: mCherry. | | cancer | cancer-genetics |
| Amano, T. et al. Controllable self-replicating RNA vaccine delivered intradermally elicits predominantly cellular immunity. iScience. 2023, 26,106335. | Minoru S.H. Ko, Elixirgen Therapeutics, Inc., Baltimore, MD, USA | Pathogenic Coronaviruses | Acute respiratory syndrome coronavirus 2 (SARS-CoV-2) - SARS-CoV - Middle East respiratory syndrome coronavirus (MERS-CoV)" | BLI | Mouse | Ami HT | 2022 | Developed an mRNA vaccine platform for skin delivery without nanoparticles. developed an srRNA that functions optimally at around 33C (skin temperature) and is inactivated at or above 37C (core body temperature) as a safety switch. This temperature-controllable srRNA (c-srRNA), when tested as an intradermal vaccine against SARS-CoV-2, functions when injected naked without lipid nanoparticles. | | pathogenic-coronaviruses | acute-respiratory-syndrome-coronavirus-2-sars-cov-2-sars-cov-middle-east-respiratory-syndrome-coronavirus-mers-cov |
| Karsten, L. et al. Bivalent EGFR-Targeting DARPin-MMAE Conjugates. International Journal of Molecular Sciences. 2022, 23: 2468. | Kristian Muller, Cellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, 33615 Bielefeld, Germany | Cancer, Squamous Cell Carcinoma and Glioma | Antibody-drug conjugates | FLI | Mouse | Lago | 2022 | Constructed protein-drug conjugates based on the anti-Epidermal growth factor receptor (EGFR). Designed Ankyrin Repeat Protein (DARPin) E01, and compared the bivalent DARPin dimer (DD1) and a DARPin-Fc (DFc) to the monomeric DARPin (DM) and the antibody derived scFv425-Fc (scFvFc) in cell culture and a mouse model. Probe: Alexa Fluor 647. | | cancer squamous-cell-carcinoma-and-glioma | antibody-drug-conjugates |
| Park, S. et al. Bioluminescence Imaging of Matrix Metalloproteinases-2 and -9 Activities in Ethanol-injured Cornea of Mice. in vivo. (2021) 35: 1521-1528. | Kyung-Jong Won, Department of Physiology, Konkuk University School of Medicine, Seoul, Republic of Korea | Ethanol-induced damage in the cornea of mice | Role of matrix metalloproteinases (MMP)-2 and MMP-9 activation | BLI | Mouse | Ami HTX | 2021 | BLI can be applied in vivo in mice with corneal injury to examine the activity of MMPs and clarify the efficacy of eye drops. | | ethanol-induced-damage-in-the-cornea-of-mice | role-of-matrix-metalloproteinases-mmp-2-and-mmp-9-activation |
| Andradas, C. et al. Assessment of Cannabidiol and Δ9-Tetrahydrocannabiol in Mouse Models of Medulloblastoma and Ependymoma. Cancers (Basel). 2021 Jan 18;13(2):330. | Clara Andradas, Brain Tumour Research Program, Telethon Kids Institute, Nedlands, WA 6009, Australia. | Cancer, Medullablastoma | Assessment of Cannabidiol and ∆9-Tetrahydrocannabiol | BLI | Mouse | Lago X | 2021 | Cannabinoids had cytotoxic activity against medulloblastoma and ependymoma cells in vitro, functioning in part through the inhibition of cell cycle progression and the induction of autophagy. Despite these effects in vitro, when tested in orthotopic mouse models of medulloblastoma or ependymoma, no impact on animal survival was observed. | | cancer medullablastoma | assessment-of-cannabidiol-and-%e2%88%869-tetrahydrocannabiol |
| Lopez-Pier, M., et al. An adaptable and non-invasive method for tracking Bifidobacterium animalis subspecies lactis 420 in the mouse gut. J Microbial Methods, 2021, 189: 106302 | John Konhilas, Department of Physiology, University of Arizona, Tucson, AZ | Gut Microbiota | Tracking bacteria in the gut | FLI | Mouse | Lago X | 2021 | Labeling of microbial species with an externally detectable marker allows detection as to their specific geographic residence/location during transit through the gut. Incubated B420 with common, FDA-approved contrast agents, ISOVUE-300 or indocyanine green (ICG), and visualized by x-ray fluoroscopy (ISOVUE-300) or fluorescence (ICG) along the GI tract at different timepoints. Validated and quantified B420 using quantitative PCR at different regions of the GI tract. ICG was a more effective labeling agent over ISOVUE-300. Probe: Indocyanine green (ICG). | | gut-microbiota | tracking-bacteria-in-the-gut |
| Choi, J., et al. A unique subset of glycolytic tumor propagating cells drives squamous cell carcinoma. Nat. Metab. 2021. 3(2): 182–195. | Raul Mostoslavsky, The Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA | Cancer, Head and Neck Squamous Cell Carcinoma | Tumor progression | BLI | Mouse | Ami HTX | 2021 | Defined a subset of Tumor Propagating Cells (TPCs) as exhibiting increased glutathione (GSH) and provided strong in vivo data for the importance of increased glycolysis in generating glutathione and defending against oxidative stress. Discovered a unique “metabolic heterogeneity” within TPCs, indicating that only a defined (previously unknown) subpopulation of CD34+ TPCs acquires metabolic adaptations that could drive tumorigenesis | | cancer head-and-neck-squamous-cell-carcinoma | tumor-progression |
| Abramson, A., et al. A flexible electronic strain sensor for the real-time monitoring of tumor regression. Science Advances, 2022, 8, eabn6650 | Zhenan Bao, 1 Department of Chemical Engineering, Stanford University, Stanford, CA | Cancer, Lung cancer | Tumor size monitoring | BLI | Mouse | Lago X | 2022 | Commercially scalable wearable electronic strain sensor that automates the in vivo testing of cancer therapeutics by continuously monitoring the micrometer-scale progression or regression of subcutaneously implanted tumors at the minute time scale. Regression measurements were validated through histology, and caliper and bioluminescence measurements | | cancer lung-cancer | tumor-size-monitoring |
| Yang, F., et al. A biomineral-inspired approach of synthesizing colloidal persistent phosphors as a multicolor, intravital light source. Science Advances, 2022, 8, eabo6743 | Guosong Hong, Department of Materials Science and Engineering, Stanford University, Stanford, CA | Optics | Nanophosphor Development | FLI | Mouse | Lago X | 2022 | Bioinspired demineralization (BID) strategy to synthesize stable colloidal solutions of solid-state phosphors in the range of 470 to 650 nm and diameters down to 20 nm. The exceptional brightness of BID-produced colloids enables their utility as multicolor luminescent tags in vivo with favorable biocompatibility. Because of their stable dispersion in water, BID-produced nanophosphors can be delivered systemically, acting as an intravascular colloidal light source to internally excite genetically encoded fluorescent reporters within the mouse brain. Probe: nanophosphor colloid | | optics | nanophosphor-development |
| Zhong, Y., et. al., Co-administration of of iRGD enhances tumor-targeted delivery and anti-tumor effects of paclitaxel-loaded PLGA nanoparticles for colorectal cancer treatment. International Journal of Nanomedicine, 2019, 14: 8543–8560. | 1-Shan Liu, and 2-Hao Yang, 1-Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, China, 2-West China Hospital, Sichuan University, Chengdu, China | Cancer, Colorectal Cancer | Nanoparticle (NP) therapeutic delivery for colorectal cancer: | FLI | Mouse | Lago X | 2019 | Nanoparticle (NP) therapeutic delivery for colorectal cancer: Tumor targeting of Paclitaxel (PTX)-loaded PLGA nanoparticles (PLGA-PTX) was enhanced by co-administered iRGD. Note: iRGD is a bifunctional peptide ligand containing a tumor-homing motif (RGD) and a tissue-penetrating motif (CendR). Once bound to tumor-associated iRGD sites, binding to CendR enhances tumor membrane permeability, elevating NP transport across membrane., Probe: DiD | | cancer colorectal-cancer | nanoparticle-np-therapeutic-delivery-for-colorectal-cancer |
| Zhong, Q., et. al., Polymeric perfluorocarbon nanoemulsions are ultrasound-activated wireless drug infusion catheters, Biomaterials, June 2020, 206: 73-86. | Raag Airan, Stanford University, Stanford, CA, USA | Therapeutic Drug Delivery | Ultrasound-activated drug-loaded nanoemulsions | FLI | Rat | Lago | 2020 | Ultrasound-activated drug-loaded nanoemulsions: Chemistries determined and a generalized production platform developed for polymeric perfluorocarbon nanoemulsions designed to cage a wide array of hydrophobic drugs, released by ultrasound signal, with a high potential for clinical translation, Probe: IR 800 | | therapeutic-drug-delivery | ultrasound-activated-drug-loaded-nanoemulsions |
| Zeiderman, M.R., et. al., Acidic pH-targeted chitosan capped mesoporous silica coated gold nanorods facilitate detection of pancreatic tumors via multispectral optoacoustic tomography. ACS biomaterials science & engineering, 2016, 2(7), 1108–1120. | Lacey R. McNally, University of Louisville, Louisville, KY, USA | Cancer, Pancreatic Cancer | Pancreatic tumor detection and treatment with novel theranostic particles | FLI | Mouse | Ami HTX | 2016 | Pancreatic tumor detection and treatment with novel theranostic particles: Authors developed an acidic pH-targeted, chitosan capped, mesoporous silica (MS), gold nanorods (MS-GNRs) as both a tumor-targeting and drug-delivering nanoparticle (NP), with a NIR signals for sensitive optoacoustic (OA) imaging. Details on NP structure: MS protected the GNRs from thermal deformation, enhanced GNR thermal stability, and increased OA signal. Tumor-specific release of test therapeutic, gemcitabine, was mediated by two NP "gate keepers:" chitosan and a variant 7 of low pH insertion proteins (V7-LpHIPs), where chitosan and V7-LpHIP changed conformation in the presence of tumor micro enviroment's low pH. NIR signals of the NPs were provided by loaded IR-780 perchlorate dye and gold nanorods, Probes: luciferase, IR-780 dye (see supplemental figures) | | cancer pancreatic-cancer | pancreatic-tumor-detection-and-treatment-with-novel-theranostic-particles |
| Yuan, G., et. al., Elevated NSD3 histone methylation activity drives squamous cell lung cancer. Nature, 2021, 590(7846), 504-508. | 1-Ning-Yi Shao, 2-Łukasz Jaremko, 3-Pawel K. Mazur, and 4-Or Gozani, 1-University of Macau, Macau SAR, China, 2-King Abdullah University of Science and Technology, Thuwal, Saudi Arabia, 3-The University of Texas MD Anderson Cancer Center, Houston, TX, USA, and 4-Stanford University, Stanford, CA, USA | Cancer, Lung cancer | Lung squamous cell carcinoma (LUSC) tumorigenesis | BLI | Mouse | Ami HTX | 2021 | Lung squamous cell carcinoma (LUSC) tumorigenesis: Histone H3 lysine 36 (H3K36) methyltransferase NSD3 (located in the 8p11–12 amplicon) was identified as a key regulator of LUSC tumorigenesis. Specifically, a LUSC-associated variant NSD3 (T1232A) showed increased catalytic activity for dimethylation of H3K36 (H3K36me2) and was found to drive LUSC tumorigenesis, Probes: luciferase, akaLuc | | cancer lung-cancer | lung-squamous-cell-carcinoma-lusc-tumorigenesis |
| Yoon, H.Y., et. al., Glycol chitosan nanoparticles as specialized cancer therapeutic vehicles: Sequential delivery of doxorubicin and Bcl-2 siRNA. Scientific Reports, 2014, 4(1), 6878. | 1-Kwangmeyung Kim and 2-Martin G. Pomper, 1-Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea, 2-Johns Hopkins School of Medicine, Baltimore, MD, USA | Cancer, Prostate Cancer | Glycol chitosan nanoparticles (CNPs) as specialized cancer therapeutic vehicles | FLI | Mouse | Ami HTX | 2014 | Glycol chitosan nanoparticles (CNPs) as specialized cancer therapeutic vehicles: Authors developed CNPs loaded with both doxorubicin (DOX) and Bcl-2 siRNA. These dual-therapeutic,loaded CNPs effectively reduced prostate tumor load in a mouse model, Probe: Cy5.5 | | cancer prostate-cancer | glycol-chitosan-nanoparticles-cnps-as-specialized-cancer-therapeutic-vehicles |
| Yong, C., et. al., Locally invasive, castrate-resistant prostate cancer in a Pten/Trp53 double knockout mouse model of prostate cancer monitored with non-invasive bioluminescent imaging. PLoS ONE, 2020, 15(9). | Michael D. Henry, University of Iowa, Iowa City, IA, USA | Cancer, Prostate Cancer | Castration resistant invasive prostate cancer | BLI | Mouse | Ami HTX | 2020 | Castration resistant, invasive prostate cancer: A Pten/Trp53 double knockout mouse model of castrate resistant, invasive prostate cancer was monitored with non-invasive bioluminescent imaging. This model was set up by employing a Probasin-Cre mediated deletion of Pten and Trp53, coupled with the activation of a ROSA-LSL luciferase reporter., Probe: luciferase | | cancer prostate-cancer | castration-resistant-invasive-prostate-cancer |
| Yin, W., et. al., Syndecan-1 tagged liposomes as a theranostic nanoparticle for pancreatic adenocarcinoma. College of Arts & Sciences, Senior Honors Theses, 2016, Paper 126. | Wenyuan Yin, University of Louisville, Louisville, KY, USA | Cancer, Pancreatic Cancer | Directed-liposome as a theranostic nanoparticle, Therapeutic delivery mechanism for pancreatic adenocarcinoma | FLI, BLI | Mouse | Ami HTX | 2016 | Directed-liposome as a theranostic nanoparticle, and a therapeutic delivery mechanism for pancreatic adenocarcinoma: A syndecan-1-tagged liposome, loaded with fluorescent dye, achieved both specific pharmacokinetics to and non-invasive detection of orthotopic pancreatic cancer, as determined by multispectral optoacoustic tomography, Probes: luciferase, CF-750 | | cancer pancreatic-cancer | directed-liposome-as-a-theranostic-nanoparticle therapeutic-delivery-mechanism-for-pancreatic-adenocarcinoma |
| Yin, W., et. al., Tumor specific liposomes improve detection of pancreatic adenocarcinoma in vivo using optoacoustic tomography, Journal of Nanobiotechnology, 2015, 13:90. | Lacey R. McNally, University of Louisville, Louisville, KY, USA | Cancer, Pancreatic Cancer | Directed-liposome as a theranostic nanoparticle, Therapeutic delivery mechanism for pancreatic adenocarcinoma | FLI, BLI | Mouse | Ami HTX | 2015 | Directed-liposome as a theranostic nanoparticle, and a therapeutic delivery mechanism for pancreatic adenocarcinoma: A syndecan-1-tagged liposome, loaded with fluorescent dye, achieved both specific pharmacokinetics to and non-invasive detection of orthotopic pancreatic cancer, as determined by multispectral optoacoustic tomography, Probes: CF-750, propidium iodide, luciferase | | cancer pancreatic-cancer | directed-liposome-as-a-theranostic-nanoparticle therapeutic-delivery-mechanism-for-pancreatic-adenocarcinoma |
| Yang, H., et. al., Endogenous IgG-based affinity-controlled release of TRAIL exerts superior antitumor effects, Theranostics, 2018, 8(9): 2459-2476. | Xiaofeng Lu, West China Hospital, Sichuan University, Chengdu, China | Cancer, Colorectal Cancer | TRAIL in vivo pharmacokinetics optimized for superior antitumor efficacy | FLI | Mouse | Lago X | 2018 | TRAIL in vivo pharmacokinetics optimized for superior antitumor efficacy: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) was fused to endogenous IgG binding domain (IgGBD) moiety to enhance serum half-life, without any steric hindrance of TRAIL binding to its apoptosis-inducing site on tumors. In vivo trials showed that IgG-based affinity-controlled release of IgBD-TRAIL, did indeed lead to greater serum half-life and anti-tumor efficacy of TRAIL moiety. , Probe: CF750 | | cancer colorectal-cancer | trail-in-vivo-pharmacokinetics-optimized-for-superior-antitumor-efficacy |
| Yadav, S., et. al., MIR155 Regulation of Ubiquilin1 and Ubiquilin2: Implications in Cellular Protection and Tumorigenesis. Neoplasia, 2017, Vol. 19, No. 4, pp. 321-332 | 1,2-Sanjay Yadav and Levi J. Beverly, 1-University of Louisville, Louisville, KY, USA, 2-CSIR-Indian Institute of Toxicology Research, Lucknow, India | Cancer, Lung cancer | miRNA-mediated loss of Ubiquilin protein expression | FLI, BLI | Mouse | Ami HT | 2017 | miRNA-mediated loss of Ubiquilin protein expression: An increase in miRNA expression (specifically MIR155) increased lung cell invasion, migration, wound formation and clonogenicity in UBQLN-loss dependent manner. Background: Pollution carcinogen, diesel exhaust particles (DEP), found to upregulate Ubiquilin expression (and suppress MIR155 expression) and so initially protect lung cells; however, authors propose that "...DEP-induced repression of MIR155 leads to increased UBQLN levels, which in turn may be a selective pressure on lung cells to lose UBQLN1.", Probes: luciferase, GFP | | cancer lung-cancer | mirna-mediated-loss-of-ubiquilin-protein-expression |
| Wu, B., et. al., Oligo(ethylene glycol)-functionalized squaraine fluorophore as a near-infrared-fluorescent probe for the in vivo detection of diagnostic enzymes. Analytical Chemistry, 2018, 90:9359-9365. | Fang Zeng and Shuizhu Wu, South China University of Technology, Guangzhou, China | Cancer, Liver Cancer | In vivo detection of tumor-associated diagnostic enzymes | FLI | Mouse | Ami HT | 2018 | In vivo detection of tumor-associated, diagnostic enzymes: Leucine aminopeptidase (LAP), expressed at high levels in hepatomas, can function as a diagnostic enzyme, where an activatable, NIR squaraine fluorophore is specifically cleaved (and so activated) by LAP. Aqueous solubility (functionality) of this squaraine fluorophore has been improved by a oligo (ethylene glycol) addition, Probe: Oligo (ethylene glycol) modified Squaraine | | cancer liver-cancer | in-vivo-detection-of-tumor-associated-diagnostic-enzymes |
| Wen, F., et. al., Extracellular DNA in pancreatic cancer promotes cell invasion and metastasis. Cancer research, 2013, 73(14), pp.4256–4266. | 1-Fushi Wen and 2-Jiaqi Shi, 1-University of Arizona, Tucson, AZ, USA, 2-University of Michigan, Ann Arbor, MI, USA | Cancer, Pancreatic Cancer | Extracellular DNA promotes tumor-associated inflammation; cell invasion and aggressive metastasis | BLI | Mouse | Ami HT | 2013 | Extracellular DNA (ExDNA) promotes tumor-associated inflammation, cell invasion and aggressive metastasis: ExDNA containing fibers from neutrophils (nicknamed neutrophil extracellular traps or NETs found on the surface of pancreatic cancer cells, believed to be involved in inflammatory positive feedback loop (promoting expression of the inflammatory chemokine CXCL8 which led to more ExDNA), tumor growth and metastases suppressed by DNase 1 treatment, Probe: luciferase | | cancer pancreatic-cancer | extracellular-dna-promotes-tumor-associated-inflammation-cell-invasion-and-aggressive-metastasis |
| Watson, J.R., et.al., Intraoperative imaging using intravascular contrast agent. Society of Photo-Optical Instrumentation Engineers, Conference Proceedings, 2016, Vol.9696. | Marek Romanowski, University of Arizona, Tucson, AZ, USA | Cancer, Glioblastoma | Intraoperative tumor detection | BLI, FLI | Rat | Lago X | 2016 | Intraoperative tumor detection: Indocyanine green (ICG) tumor uptake by enhanced permeability and retention effect (EPR). Tumor surgical resection guided by ICG NIR fluorescent signal as viewed by augmented microscopy. ICG NIRF confirmed by bioluminescent signal of Luc-C6 glioblastoma used in rat model, Probes: luciferase, ICG | | cancer glioblastoma | intraoperative-tumor-detection |
| Varzavand, A., et. al., α3β1 Integrin Suppresses Prostate Cancer Metastasis via Regulation of the Hippo Pathway. Cancer Research, 2016, 76(22), 6577–6587. | Michael D. Henry and Christopher S. Stipp, University of Iowa, Iowa City, IA, USA | Cancer, Prostate Cancer | Prostate cancer metastasis regulation | BLI, FLI | Mouse | Ami X | 2016 | Prostate cancer metastasis regulation: Have identified an a3 integrin-Abl kinase-Hippo suppressor pathway in prostate cancer in which a3b1 integrin signals through Abl kinases to restrain Rho GTPase activity, sustain Hippo suppressor functions,and curtail metastatic cell phenotypes., Probes: luciferase, GFP | | cancer prostate-cancer | prostate-cancer-metastasis-regulation |
| Tao, Z., et. al., Targeted delivery to tumor-associated pericytes via an affibody with high affinity for PDGFRβ enhances the in vivo antitumor effects of human TRAIL. Theranostics, 2017, 7(8): 2261-2276. | Xiaofeng Lu, West China Hospital, Sichuan University, Chengdu, China | Cancer, Colon Cancer | Tumor-targeted hTRAIL therapy | FLI | Mouse | Lago X | 2017 | Tumor-targeted hTRAIL therapy: Human tumor necrosis factor-related apoptosis-inducing ligand (hTRAIL) was fused to an affibody with high affinity for platelet-derived growth factor receptor β (PDGFRβ), which is expressed by pericytes that are enriched in tumor tissues. This affibody-mediated binding of hTRAIL to tumor cells did not interfere with hTRAIL's death receptor binding and activation, and indeed, tumor hTRAIL uptake and antitumor effect was increased in colorectal xenografts in mice, Probe: CF750 | | cancer colon-cancer | tumor-targeted-htrail-therapy |
| Tang, A.C., et. al. Combination therapy with proteasome inhibitors and TLR agonists enhances tumour cell death and IL-1β production. Cell Death and Disease, 2018, 9:162. | Stuart E. Turvey, University of British Columbia, Vancouver, BC, Canada | Cancer, haematological malignancies | Combination therapy of proteasome inhibitors and Toll-like receptor agonists | BLI | Mouse | Ami HTX | 2018 | Combination therapy of proteasome inhibitors and TLR agonists: enhances tumour cell death and IL-1β production, Probe: GFP-luciferase | | cancer haematological-malignancies | combination-therapy-of-proteasome-inhibitors-and-toll-like-receptor-agonists |
| Sukumar, U.K., et. al., SP94-targeted triblock copolymer nanoparticle delivers thymidine kinase-p53-nitroreductase triple therapeutic gene and restores anticancer function against hepatocellular carcinoma in vivo. ACS Applied Material Interfaces, March 2020, 12(10): 11307-11319. | Ramasamy Paulmurugan, Stanford University School of Medicine, Palo Alto, CA, USA | Cancer, Hepatocellular Carcinoma | Gene-directed enzyme–prodrug therapy (GDEPT) | BLI, FLI | Mouse | Lago | 2020 | Gene-directed enzyme–prodrug therapy (GDEPT): Used triblock copolymer NPs: Polyethylenimine (PEI) conjugated with poly(D,L-lactic-co-glycolic acid) (PLGA) and poly(ethylene glycol) (PEG) for non-toxic delivery of negative nucleic acids, and for delivery of a rationally engineered thymidine kinase (TK)–p53–nitroreductase (NTR) triple therapeutic to restore p53 function and potentiate cancer cell response to delivered prodrugs (ganciclovir (GCV) and CB1954) with expression of suicide genes (TK,NTR). Hepatocellular carcinoma (HCC) tumor-targeting of NPs achieved with addition of SP94 peptide, Probes: luciferase, CytoCy5 | | cancer hepatocellular-carcinoma | gene-directed-enzyme-prodrug-therapy-gdept |
| Sukumar, U.K., et. al., Intranasal delivery of targeted polyfunctional gold-iron oxide nanoparticles loaded with therapeutic microRNAs for combined theranostic multimodality imaging and presensitization of glioblastoma to Temozolomide. Biomaterials, October 2019, 218. | 1-Tarik F. Massoud and 2-Ramasamy Paulmurugan, 1-Stanford University School of Medicine, Stanford, CA, USA, 2-Stanford University School of Medicine, Palo Alto, CA, USA | Cancer, Glioblastoma | Theranostic; intranasal and systemic combinatorial therapy of GBM | BLI | Mouse | Lago X | 2019 | Theranostic, intranasal and systemic combinatorial therapy for GBM: use intranasal delivery of theranostic, polyfunctional gold-iron oxide nanoparticles (polyGIONs) surface-loaded with therapeutic Cy5-miRNAs (miR-100 and antimiR-21), along with systemically delivered chemotherapy drug temozolomide (TMZ), to treat orthotopic mouse model of FLUC-EGFP labeled U87-MG GBMs, Probes: luciferase, Cy5 | | cancer glioblastoma | theranostic-intranasal-and-systemic-combinatorial-therapy-of-gbm |
| Suhardi, V.J., et. al., A Fully Functional Drug-Eluting Joint Implant. Nature biomedical engineering, 1(6), pp.Suhardi, VJ, DA Bichara, SJJ Kwok, AA Freiberg, H Rubash, H Malchau, SH Yun, OK Muratoglu, and E Oral. Nat Biomed Eng. 2017, 1. | 1,2-E Oral, 1-Harris Orthopaedic Laboratory, Massachusetts General Hospital, Boston, MA, USA, and 2-Harvard Medical School, Cambridge, MA, USA | Revision of Infected Prosthetic Joints | Antibiotic drug delivery and mechanical strength of prosthetic joint implant material | BLI | Rabbit | Ami HTX | 2017 | Optimize joint implant antibiotic drug delivery and mechanical strength: Modified eccentricity ofdrug clusters and percolation threshold in ultrahigh molecular weight polyethylene (UHMWPE) to maximize drug elution rate while retaining UHMWPE mechanical strength. Lapine knees infected with Staphylococcus aureus with the antibiotic-eluting UHMWPE led to complete bacterial eradication and to the absence of detectable systemic effects, outperforming the current clinical gold standard model of antibiotic-eluting bone cement joint construct, Probe: luciferase | | revision-of-infected-prosthetic-joints | antibiotic-drug-delivery-and-mechanical-strength-of-prosthetic-joint-implant-material |
| Su, Y., et. al., Novel NanoLuc substrates enable bright two-population bioluminescence imaging in animals, Nature Methods, 2020, 17, 852–860. | 1-Thomas A. Kirkland, and 2-Michael Z. Lin, 1-Promega Biosciences LLC, San Luis Obispo, CA, USA, 2-Stanford University, Stanford, CA, USA | Bioluminescent Imaging | Dual Bioluminescence Imaging | BLI | Mouse | Ami HT | 2020 | New Nanoluc substrate enhances dual in vivo bioluminescence imaging: Previously, Antares, a fusion of NanoLuc to the orange fluorescent protein CyOFP (an example of BRET) used with AkaLuc and its substrate AkaLumine to provide a dual biolum, as the two luciferases have orthogonal substrate specificity. NanoLuc bioluminescence brightness has been improved: first, with hydro-furimazine, and then even more so with fluorofurimazine. Used Antares with fluorofurimazine to track tumor size, AkaLuc/Akalumine to visualize CAR-T cells within the same mice, Probes: Antares, AkaLuc | | bioluminescent-imaging | dual-bioluminescence-imaging |
| Stokes, J., et. al., Post‐transplant bendamustine reduces GvHD while preserving GvL in experimental haploidentical bone marrow transplantation. British Journal of Haematology, 2016, 174(1), 102–116. | Emmanuel Katsanis, University of Arizona, Tucson, AZ, USA | Cancer, Lymphoma | GVHD prevention and GVL preservation | BLI | Mouse | Lago | 2016 | GVHD prevention and GVL preservation: in cases of haploidentical bone marrow transplantation (h-BMT), achieved by post-transplant bendamustine treatment, Probe: luciferase | | cancer lymphoma | gvhd-prevention-and-gvl-preservation |
| Song, J.H. & Kraft, A.S., Insulin receptor substrate 1 is a key substrate for Pim protein kinases. Cancer Research, 2016, 76(s14), 4414–4414. | Jin H. Song, and Andrew S. Kraft, University of Arizona, Tucson, AZ, USA | Cancer, Lymphomas | Pim Kinase substrates and associated regulatory pathways | BLI | Mouse | Lago X | 2016 | Haematopoietic malignancies: Pim family of serine/threonine protein kinases (Pim 1, 2, and 3) contribute to cellular transformation by regulating glucose metabolism, protein synthesis, and mitochondrial oxidative phosphorylation. Therefore, aim to identify Pim substrates to determine pathways regulated by enzymes, and to have substrates serve as possible biomarkers of Pim kinase activity, Probe: luciferase | | cancer lymphomas | pim-kinase-substrates-and-associated-regulatory-pathways |
| Shin, J.M., et. al., A carboxymethyl dextran-based polymeric conjugate as the antigen carrier for cancer immunotherapy. Biomaterials Research, 2018, 22:21. | Jae Hyung Park, Sungkyunkwan University, Suwon, Republic of Korea | Cancer, Cervical Cancer | Immunotherapy by polymeric antigen carrier | FLI | Mouse | Lago X | 2018 | Immunotherapy by polymeric antigen carrier: Carboxymethyl dextran (CMD) used as the polymeric backbone and ovalbumin (OVA)as a model foreign antigen, CMD-OVA conjugate successfully taken up by TC-1 cervical cancer in vivo, , Probe: Cy5.5 | | cancer cervical-cancer | immunotherapy-by-polymeric-antigen-carrier |
| Shi, Q., et. al., PDGFR beta-specific affibody-directed delivery of a photosensitizer, IR700, is efficient for vascular-targeted photodynamic therapy of colorectal cancer. Drug Delivery, 2017, 24(1), pp.1818–1830. | Xiaofeng Lu, West China Hospital, Sichuan University, Chengdu, China | Cancer, Colorectal Cancer | Photodynamic therapy | FLI | Mouse | Lago X | 2017 | Photodynamic therapy (PDT) specificity enhanced by targeting vascular pericyte membrane protein, PDGFRb: Photosensitizer, IR700, conjugated to affibody directed against PDGFRb, showed binding specificity to tumor vascular PDGFRb in vivo. Ensuing PDT intensitied tumor specific hypoxia, and reduced in tumor mass vs. negative controls. Additionally, above PDT led to increased tumor uptake of TNF-related apoptosis-inducing ligand (TRAIL) injected post-illumination. This dual therapy showed greater tumor suppression than either mono-therapy, Probe: IR700 | | cancer colorectal-cancer | photodynamic-therapy |
| Shankar, G.M., et. al., Genotype-targeted local therapy of glioma. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115(36), E8388–E8394. | 1,2-Giovanni Traverso, and 3-Daniel P. Cahill, 1-Massachusetts Institute of Technology, Cambridge, MA, USA, 2-Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA, and 3-Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA | Cancer, Glioblastoma | Genotype-targeted therapy | BLI | Mouse | Ami HTX | 2018 | Genotype-targeted therapy of low grade gliomas: Given that most low grade gliomas harbor isocitrate dehydrogenase 1 (IDH1) or IDH2 mutations, they are sensitive to metabolism-altering agents. Developed and tested an intraoperative treatment that coupled both a rapid multiplexed genotyping for IDH mutant gliomas (by qPCR following resection) with, if positive, a sustained release of IDH-directed nicotinamide phosphoribosyltransferase (NAMPT) inhibitor from loaded microparticles (MPs) as a locally toxic therapy, Probe: luciferase | | cancer glioblastoma | genotype-targeted-therapy |
| Schüler, E., et. al., Experimental Platform for Ultra-high Dose Rate FLASH Irradiation of Small Animals Using a Clinical Linear Accelerator. International Journal of Radiation Oncology, Biology, Physics, 2017, 97(1), 195–203. | Peter G. Maxim, Stanford University School of Medicine, Stanford, CA, USA | Cancer, Radiation Oncology | protocol development, Ultra-high dose rate irradiation (>50 Gy/s “FLASH”) | XRAY | Mouse | Ami HTX | 2017 | New radiation therapy technique with potentially minimal normal tissue toxicity: Protocol development of ultra-high dose rate irradiation (>50 Gy/s, “FLASH”) electron small animal irradiation with a clinically available linear accelerator (LINAC), Probe: none, X-ray imaging | | cancer radiation-oncology | protocol-development ultra-high-dose-rate-irradiation-50-gy-s-flash |
| Samykutty, A., et. al., Osteopontin-targeted probe detects orthotopic breast cancers using optoacoustic imaging. Biotech Histochemistry, 2018, 93(8), 608-614. | Lacey R. McNally, Wake Forest School of Medicine, Winston-Salem, NC, USA | Breast Cancer, Cancer | Detection and monitoring of breast cancer by a osteopontin-NIR fluorophore conjugate | FLI | Mouse | Ami HT | 2018 | Breast cancer detection and monitoring: Use of a osteopontin-NIR fluorophore conjugate. Multispectral optoacoustic tomography (MSOT), confirmed by NIRF imaging, confirmed probe's in vivo specificity for breast tumor in mouse model , Probe: Hilyte 750 succinyl ester dye | | breast-cancer cancer | detection-and-monitoring-of-breast-cancer-by-a-osteopontin-nir-fluorophore-conjugate |
| Rice, M.A., et. al., Loss of Notch1 activity inhibits prostate cancer growth and metastasis and sensitizes prostate cancer cells to antiandrogen therapies. Molecular Cancer Therapeutics, 2019, 18(7): 1230–1242. | Tanya Stoyanova, Canary Center at Stanford for Cancer Early Detection, Stanford University, Palo Alto, CA, USA | Cancer, Prostate Cancer | NOTCH1 and Notch1 activity as therapeutic targets of aggressive prostate cancer | BLI | Mouse | Lago | 2019 | Prostate cancer: Notch1 as a therapeutic target: Loss of NOTCH1 in aggressive prostate cancer led to decreases in proliferation, invasion, tumorsphere formation, and metastatic potential. Inhibitors of Notch1 activity by Gamma secretase inhibitors RO4929097or DAPT further reduced prostate tumor cell proliferation. Loss of NOTCH1 combined with inhibitor therapies could delay development of castration-resistant prostate cancer (CRPC) and metastic events., Probe: luciferase | | cancer prostate-cancer | notch1-and-notch1-activity-as-therapeutic-targets-of-aggressive-prostate-cancer |
| Qiu, X.Y., et. al., PD-L1 confers glioblastoma multiforme malignancy via Ras binding and Ras/Erk/EMT activation. BBA – Molecular Basis of Disease, 2018, 1864: 1754-1769 | Xiao Qian Chen, Huazhong University of Science and Technology, Wuhan, China | Cancer, Glioblastoma | Programmed cell death ligand-1 (PD-L1) expression level correlated with glioblastoma multiforme malignancy | FLI | Mouse | Lago X | 2018 | Programmed cell death ligand-1 (PD-L1) expression level correlated with glioblastoma multiforme maligency: PD-L1 binding to intracellular Ras leads to enhanced GBM malignancy via activating Ras-Erk-EMT (epithelial mesenchymal transition) axis signaling. 1. And PD-L1 knockdown (by lentiviral infection (LV-sh-PD-L1, with EGFP tag)) abolished GBM development, Probe: eGFP | | cancer glioblastoma | programmed-cell-death-ligand-1-pd-l1-expression-level-correlated-with-glioblastoma-multiforme-malignancy |
| Phuong, P.T.T., et. al., Beta-carotene-bound albumin nanoparticles modified with chlorin e6 for breast tumor ablation based on photodynamic therapy. Colloids and Surfaces B: Biointerfaces, 2018, 171: 123-133. | Beom Soo Shina, and Yu Seok Youn, Sungkyunkwan University, Suwon, Republic of Korea | Breast Cancer, Cancer | Breast tumor ablation by photodynamic therapy | FLI | Mouse | Lago X | 2018 | Breast tumor ablation by photodynamic therapy: Chlorin e6 (Ce6) used as a 2nd generation photosynthesizer therapeutic and fluorophore probe, and Ce6 water solubility was improved with "nab" (nanoparticle albumin-bound) technology, including beta-carotene as a cross-linker in the final, loaded albumin NPs, Ce6-BSA-BC-NPs, which showed efficacy vs 4T1 breast tumor xenografts following 660 nm laser irradiation in a mouse model, Probe Ce6 | | breast-cancer cancer | breast-tumor-ablation-by-photodynamic-therapy |
| Park, S.B., et. al., Bioluminescence imaging of matrix metalloproteinases-2 and -9 activities in ethanol-injured cornea of mice. In vivo, 2021, 35: 1521-1528. | Kyung-Jong Won, Konkuk University School of Medicine, Seoul, Republic of Korea | Eye Corneal Injury and Healing | Non-invasive monitoring eye corneal injury and healing | FLI | Mouse | Ami HTX | 2021 | Non-invasive monitoring eye corneal injury and healing: following 20% ethanol treatment, the extent of eye (corneal epithelial) apoptosis and inflammation was assessed by matrix metalloproteinases (MMP)-2 and MMP-9 activation and activity, as indicated by fluorescence signal intensity of activatable probe NpFlamma® MMP-2/9; eye drops (hyaluronic acid and serum) efficacy was noted by a reduction in NpFlamma® MMP-2/9 signal, Probe: NpFlamma® MMP-2/9 | | eye-corneal-injury-and-healing | non-invasive-monitoring-eye-corneal-injury-and-healing |
| Park, S., et. al., Gold nanocluster-loaded hybrid albumin nanoparticles with fluorescence-based optical visualization and photothermal conversion for tumor detection/ablation. Journal of Controlled Release, 2019, 304: 7-18. | Yu Seok Youn, Sungkyunkwan University, Suwon, Republic of Korea | Cancer, Colon Cancer | Tumor Detection and Ablation by hybrid gold nanoclusters (AuNCs) | FLI | Mouse | Lago X | 2019 | Tumor Detection and Ablation by hybrid gold nanoclusters (AuNC): Smaller AuNC (<1-2 nm) allow Cy5.5 fluorescence, Larger AuNC (several nm in size) are good hyperthermic agents. Together, as a hybrid population of AuNCs, with albumin (AuNCs/BSA-NPs), tumor specific uptake, detection, and ablation (by laser therapy) is achieved in a colon cancer model, Probe: Cy 5.5 | | cancer colon-cancer | tumor-detection-and-ablation-by-hybrid-gold-nanoclusters-auncs |
| Park, J., et. al., Magnetophoretic delivery of a tumor priming agent for chemotherapy of metastatic murine breast cancer. Molecular Pharmaceuticals, 2019 May, 16(5): 1864-1873. | Yoon Yeo, Purdue University, West Lafayette, IN , USA | Breast Cancer, Cancer | Improving NP delivery to tumor micro environment (TME) | FLI | Mouse | Ami HT | 2019 | Primary tumor pretreatment with iron-oxide Nanoparticles: Facilitate penetration into the tumor microenvironment (TME), without elevating occurrences of metastases, by the timely application of paclitaxel (PTX)-loaded iron oxide decorated poly(lactic-co-glycolic acid) nanoparticle composite (PTX@PINC) along with an external magnet, prior to secondary NP treatment, Probe: DiR | | breast-cancer cancer | improving-np-delivery-to-tumor-micro-environment-tme |
| Pandurangi, R.S., et. al., A priori activation of apoptosis pathways of tumor (AAAPT) technology: Development of targeted apoptosis initiators for cancer treatment, PLoS ONE, 2021, 16(2). | Raghu S. Pandurangi, Sci-Engi-Medco Solutions Inc., St Charles, Missouri, USA | Cancer, various | A priori activation of apoptosis pathways of tumor technology (AAAPT) | BLI | Mouse | Lago | 2021 | A priori activation of apoptosis pathways of tumor technology (AAAPT): to activate specific cell death pathways and inhibit survival pathways, simultaneously, and to act selectively in cancer cells sparing normal cells. Tested novel therapeutic AMP-001-003 vs. MDA-MB-231 (triple negative breast cancer), PC3 (prostate cancer) and A543 (lung cancer) cells, Probe: luciferase | | cancer various | a-priori-activation-of-apoptosis-pathways-of-tumor-technology-aaapt |
| Padi, S.K.R., et. al., Targeting the PIM protein kinases for the treatment of a T-cell acute lymphoblastic leukemia subset. Cancer Research, 2017, 77(s13): 5820–5820. | Andrew S. Kraft, University of Arizona, Tucson, AZ, USA | Cancer, T-cell Acute Lymphoblastic Leukemias | pan-PIM Protein Kinase Inhibitor and Ponatinib vs.T-cell acute lymphoblastic leukemia subgroup | BLI | Mouse | Lago X | 2017 | T-cell acute lymphoblastic leukemia: Treatment by pan-PIM protein kinase inhibitors vs. T-cellacute lymphoblastic leukemia (T-ALL) subset, early T-cell precursors (ETPs) with elevated PIM1 expression (and no NOTCH mutation), and combinatorial treatment with Ponatinib, a broadly active tyrosine kinase inhibitor, led to significantly better efficacy, Probe: luciferase | | cancer t-cell-acute-lymphoblastic-leukemias | pan-pim-protein-kinase-inhibitor-and-ponatinib-vs-t-cell-acute-lymphoblastic-leukemia-subgroup |
| O’Leary, M.P., et. al., Novel oncolytic chemeric orthopoxvirus causes regression of pancreatic cancer xenografts and exhibits abscopal effect at a single low dose. Journal of Translational Medicine, 2018, 16:110. | Nanhai G. Chen, City of Hope National Medical Center, Duarte, CA, USA | Cancer, Pancreatic Cancer | Oncolytic viral therapy vs. Pancreatic Cancer | BLI | Mouse | Lago X | 2018 | Oncolytic Chimeric Orthopoxvirus: Recombinant virus encoding firefly luciferase, CF33-Fluc, specific uptake, replication in, and lysis of pancreatic tumors in PANC-1 and MIA PaCa-2 xenograft models., Probe: luciferase | | cancer pancreatic-cancer | oncolytic-viral-therapy-vs-pancreatic-cancer |
| O’Leary, M.P., et. al., A novel oncolytic chimeric orthopoxvirus encoding luciferase enable real-time view of colorectal cancer cell infection. Molecular Therapy Oncolytics, June 2018, 9: 13-21. | Nanhai G. Chen, City of Hope National Medical Center, Duarte, CA, USA | Cancer, Colorectal Cancer | Oncolytic viral therapy vs. Colorectal Cancer | BLI | Mouse | Lago X | 2018 | Oncolytic Chimeric Orthopoxvirus: Recombinant virus encoding firefly luciferase, CF33-Fluc, specific uptake, replication in, and lysis of HCT-116 colorectal tumor xenograft in mouse model., Probe: luciferase | | cancer colorectal-cancer | oncolytic-viral-therapy-vs-colorectal-cancer |
| O’Leary, B.R., et. al., Pharmacological ascorbate inhibits pancreatic cancer metastases via a peroxide-mediated mechanism. Nature Research Scientific Reports, 2020, 10: 17649 | Joseph J. Cullen, and Michael D. Henry, University of Iowa, Iowa City, IA, USA | Cancer, Pancreatic Cancer | Pharmacological ascorbate treatment of pancreatic ductal adenocarcinoma (PDAC) | BLI | Mouse | Ami HTX | 2020 | Pharmacological ascorbate (high-dose, intravenous vitamin C): cytotoxic specifically to pancreatic ductal adenocarcinoma (PDAC), it prevents PDAC metastases by inducing oxidative stress specifically in PDAC cells, not in normal somatic cells; mechanism: hydrogen peroxide production by auto oxidation of pharmacological ascorbate; observed a reduction in circulating tumor cell (CTC) nucleases following ascorbate treatment , Probe: luciferase | | cancer pancreatic-cancer | pharmacological-ascorbate-treatment-of-pancreatic-ductal-adenocarcinoma-pdac |
| O’Leary, B.R., et. al., Loss of SOD3 (EcSOD) Expression Promotes an Aggressive Phenotype in Human Pancreatic Ductal Adenocarcinoma, AARC, Clinical Cancer Research, January 29, 2015. | James J. Mezhir, University of Iowa, Iowa City, IA, USA | Cancer, Pancreatic Cancer | Extracellular Superoxide Dismutase (SOD) as therapeutic target for treatment of pancreatic adenocarcinoma (PDA) | BLI | Mouse | Ami HT | 2015 | Pancreatic ductal adenocarcinoma (PDA): cells lines overexpressing extracellular superoxide dismutase (EcSOD) demonstrated slower growth rates, and loss of EcSOD leading to increase in superoxide and nitric oxide interaction, lead to invasive phenotype supporting aggressive and refractory nature of PDA; treatment with SOD mimetics could be clinically effective vs. PDA, Probe: luciferase | | cancer pancreatic-cancer | extracellular-superoxide-dismutase-sod-as-therapeutic-target-for-treatment-of-pancreatic-adenocarcinoma-pda |
| Nishihara, R., et. al., Highly bright and stable NIR-BRET with blue-shifted coelenterazine derivatives for deep-tissue imaging of molecular events in vivo. Theranostics, 2019, 9(9): 2646-2661. | Koji Suzuki, Keio University, Kohoku-ku, Yokohama, Kanagawa, Japan | Breast Cancer, Cancer | NIR-BRET probes for in vivo monitoring of tumor metastases, protein-protein interactions | BLI,FLI,BRET | Mouse | Lago | 2019 | Bright and stable NIR-BRET in vivo probe: Emission peak of coelenterazine (CTZ) derivatives tuned to Soret band (blue light absorption peak) of iRFP, this NIR-BRET conjugate, encoded in mammalian plasmid, was expressed in human breast cancer (MDA-MB231) cells, and detection of cells was evaluated in mouse in model, NIR-BRET probe to be used for detection of metastases and protein-protein interactions, Probes: coelenterazine derivative (RLuc8.6-535SG) combined with iRFP | | breast-cancer cancer | nir-bret-probes-for-in-vivo-monitoring-of-tumor-metastases protein-protein-interactions |
| Moose, D.L., et. al., Cancer Cells Resist Mechanical Destruction in Circulation via RhoA/Actomyosin-Dependent Mechano-Adaptation. Cell Reports, 2020, 30: 1-11. | Michael D. Henry,University of Iowa, Iowa City, IA, USA | Cancer, Prostate Cancer | Mechanism of Resilience of circulating tumor cells (CTCs) | BLI | Mouse | Ami HTX | 2020 | Resilience of circulating tumor cells (CTCs): exhibit a mechano-adaptive response to fluid shear stress, involves activation of RhoA-actomyosin signaling axis, this signalling is shown to support intravascular survival of CTCs, Probe: luciferase | | cancer prostate-cancer | mechanism-of-resilience-of-circulating-tumor-cells-ctcs |
| Meng, F., et. al., Quantitative assessment of nanoparticle biodistribution by fluorescence imaging, revisited, ACS Nano, 2018, July 24, 12(7): 6458-6468. | Yoon Yeo, Purdue University, West Lafayette, IN , USA | Fluorescence Imaging | Fluorescence signal quenching in fluorophore-loaded nanoparticles | FLI | Mouse | Ami HT | 2018 | Fluorescence signal quenching in nanoparticles (NPs) loaded with fluorophore: DiR-loaded poly(lactic-co-glycolic acid) (PLGA) NPs show inverse correlation between DiR concentration/NP and signal strength, Probe: DiR | | fluorescence-imaging | fluorescence-signal-quenching-in-fluorophore-loaded-nanoparticles |
| Marecic, O., et al., Identification and characterization of an injury-induced skeletal progenitor. Proceedings of the National Academy of Sciences, 2015, 112(32): 9920–9925. | Irving L. Weissman, Charles K. F. Chan, and Michael T. Longaker, Stanford University School of Medicine, Stanford, CA, USA | Bone Growth, Repair | cartilage, Distinct gene expression and phenotype of fracture-induced bone, stromal progenitor (f-BCSP) cells | XRAY | Mouse | Ami HTX | 2015 | Fracture-induced bone, cartilage, stromal progenitor (f-BCSP) cells: Gene expression pattern and phenotype are similar to that in perinatal skeletogenesis, but distinct from BCSP cells from uninjured bone, f-BCSP cells may be functionally stratified, containing distinct subsets responsible for growth, regeneration, and repair, Probe: none, X-ray imaging | | bone-growth repair | cartilage distinct-gene-expression-and-phenotype-of-fracture-induced-bone stromal-progenitor-f-bcsp-cells |
| Marchal, M.A., et. al., Abl kinase deficiency promotes AKT pathway activation and prostate cancer progression and metastasis. bioRxiv, 2020. | Michael D. Henry, Christopher S. Stip, University of Iowa, Iowa City, IA, USA | Cancer, Prostate Cancer | Metastatic castration-resistant prostate cancer (mCRPC) | BLI | Mouse | Ami HT | 2020 | Prostate Cancer: metastatic castration resistant prostatic cancer, Alb deficient cell line showed elevated growth rate associated with AKT pathway activation, Abl kinase inhibitor mimicked Alb deficient cells, inhibitor of AKT pathway reduced tumor growth rate, Probe: luciferase | | cancer prostate-cancer | metastatic-castration-resistant-prostate-cancer-mcrpc |
| Lucero-Acuña A., et. al., Nanoparticle delivery of an AKT/PDK1 inhibitor improves the therapeutic effect in pancreatic cancer. International Journal of Nanomedicine, 2014,1: 5653–5665. | Emmanuelle J. Meuillet, University of Arizona, Tucson, AZ, USA | Cancer, Pancreatic Cancer | Overcoming Severe Desmoplasia | BLI | Mouse | Ami HT | 2014 | Pancreatic Cancer Desmoplasia: Severe in pancreatic cancer cell strains with K-Ras mutation, improve therapeutic delivery (e.g. PH-427) by poly (lactic-co-glycolic acid) nanoparticles (PNP), Probe: luciferase | | cancer pancreatic-cancer | overcoming-severe-desmoplasia |
| Liu, M., et. al., Dectin-1 Activation by a Natural Product β-Glucan Converts Immunosuppressive Macrophages into an M1-like Phenotype. Journal of immunology (Baltimore, Md. : 1950), 2015, 195(10): 5055–5065. | Jun Yan, University of Louisville, Louisville, KY, USA | Cancer, Lung cancer | M2 to M1 activated macrophage conversion | Dual FLI | Mouse | Ami HT | 2015 | Lung Cancer: Immunosuppressive M2 tumor associated macrophages (TAMs) and bone marrow derived macrophages (BMMs) converted to M1 tumoridical MOs by B-glucan treatment, Probes: XenoLight DiR and VivoTrack 680 | | cancer lung-cancer | m2-to-m1-activated-macrophage-conversion |
| Ling, Q., et. al., The prognostic relevance of primary tumor location in patients undergoing resection for pancreatic ductal adenocarcinoma. Oncotarget, 2017, 8(9): 15159–15167. | 1-Holger Kalthoff, and 2,3-Shusen Zheng, 1-Comprehensive Cancer Center North, CAU, Kiel, Germany, 2-College of Medicine, Zhejiang University, Hangzhou, China, and 3-Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, China | Cancer, Pancreatic | Associated miRNA expression on PDAC recurrence, Prognostic relevance of primary PDAC location | BLI | Mouse | Ami HT | 2017 | Pancreatic ductal adenocarcinoma (PDAC): Recurrence rates of PDAC following early stage resection appear, by clinical data, to be a function primary PDAC location, with body/tail cancer being a less malignant phenotype than head cancer; deregulation (elevated) expression of miRNA (miR-501-3p) promotes PDAC recurrence and metastases, Probe: luciferase | | cancer pancreatic | associated-mirna-expression-on-pdac-recurrence prognostic-relevance-of-primary-pdac-location |
| Li, Y., et. al., Intestinal helminths regulate lethal acute graft-versus-host disease and preserve the graft-versus-tumor effect in mice. Journal of Immunology, 2015, 194(3): 1011-1020. | M. Nedim Ince, University of Iowa, Iowa City, IA, USA | Cancer, Lymphoma | GVHD prevention and GVT preservation by helminth injection associated induction of Tregs | BLI | Mouse | Ami HT | 2015 | Graft vs. Host Disease (GVHD) prevention and Graft vs. Tumor (GVT) immunity preservation: achieved with helminth, H. polygyrus oral injection, leading to activation of regulatory T cells (Tregs) that modulate effector T cell associated intestinal inflammation, and any intestinal bowel disease (IBD), B cell lymphoma burden tracked by Luciferase-expressing A20 (A20-luc) lymphoma cells, Probe: luciferase | | cancer lymphoma | gvhd-prevention-and-gvt-preservation-by-helminth-injection-associated-induction-of-tregs |
| Li, S., et. al., Near infrared fluorescent imaging of brain tumor with !R780 dye incorporated phospholipid nanoparticles, Journal of Translational Medicine, 2017, 15:18 | 1-Shihong Li, and 1,2-Qian Xie, 1-Van Andel Research Institute, Grand Rapids, MI, USA, and 2-East Tennessee State University, Johnson City, USA | Cancer, Glioblastoma | Image guided oncological surgery, Tumor Detection | FLI | Mouse | Ami HT | 2017 | Glioblastoma tracking: Phospholipid micelle and liposome NPs used to deliver NIR fluorescent probe IR780 through blood brain barrier, for accumulation in orthotopic glioblastoma cells by EPR effect, Probe: IR780 | | cancer glioblastoma | image-guided-oncological-surgery tumor-detection |
| Lee, S.Y., et. al., Esterase-sensitive cleavable histone deacetylase inhibitor-coupled hyaluronic acid nanoparticles for boosting anticancer activities against lung adenocarcinoma. Biomaterials Science, 2019. | Hyun-Jeong Ko, and Hyun-Jong Cho, Kangwon National University, Chuncheon, Republic of Korea | Cancer, Lung cancer | Nanoparticle drug delivery optimization | FLI | Mouse | Ami HT | 2019 | Lung adenocarcinoma treatment: Biocompatible, biodegradable, hyaluronic acid (HA), a disaccharide, along with 4-phenylbutyric acid (PBA), with hydrophobic and hydrophilic moieties, used to build PBAHA NPs for loading curcumin (CUR), HA binding to CD44 and CD168 used as active tumor targeting, combined antitumor activities of PBA and CUR tested, NPs tracked with Cy 5.5, Probe: Cy5.5 | | cancer lung-cancer | nanoparticle-drug-delivery-optimization |
| Lee, S.Y., et. al., An α-tocopheryl succinate enzyme-based nanoassembly for cancer imaging and therapy. Drug Delivery, 2018, 25(1): 738-749. | Hyun-Jong Cho, Kangwon National University, Chuncheon, Republic of Korea | Breast Cancer, Cancer | Nanoparticle enzyme-based therapeutic | FLI | Mouse | Lago | 2018 | Breast Cancer treatment: enzyme-based nanocarrier therapeutic, nano assembly (NA) of D-a-tocopherol succinate conjugated to lysozyme enzyme, loaded with curcumin, tumor specificity is passive by enhanced permeability and retention (EPR) effect, reduced tumor growth, induced apoptosis via mitochondrial destabilization, Probe: Cy5.5 | | breast-cancer cancer | nanoparticle-enzyme-based-therapeutic |
| Lee, S.Y., et. al., Transient aggregation of chitosan-modified poly(d,l-lactic-co-glycolic) acid nanoparticles in the bloodstream and improved lung targeting efficiency. Journal of Colloid and Interface Science, 2016, 480: 102–108. | 1-In-Soo Yoon, and 2-Hyun-Jong Cho, 1-Mokpo National University, Jeonnam, Republic of Korea, and 2-Kangwon National University, Chuncheon, Republic of Korea | Cancer, Lung cancer | Nanoparticle drug delivery optimization | FLI | Mouse | Lago X | 2016 | Lung Cancer: Optimized drug delivery: use of vascular, transient aggregation of chitosan-modified poly(D,L-lactic-co-glycolic) acid nanoparticles,CS-coated PLGA NPs, Probe: Cy5.5 | | cancer lung-cancer | nanoparticle-drug-delivery-optimization |
| Lee, J.J., et. al., Predictive modeling of in vivo response to gemcitabine in pancreatic cancer. PLoS Computational Biology, 2013, 9(9). | Lacey R. McNally, University of Louisville, Louisville, KY, USA | Cancer, Pancreatic Cancer | Predictive limits of in vitro drug efficacy studies | BLI | Mouse | Ami HTX | 2013 | Pancreatic Cancer: predictive modeling of in vivo response to gemcitabine: inefficient vascularization and abundant stroma of tumor microenvironment lead to heterogeneous cell proliferation and death, and ensuing poor penetration and efficacy of gemcitabine treatment, Probe: luciferase | | cancer pancreatic-cancer | predictive-limits-of-in-vitro-drug-efficacy-studies |
| Lartey, F.M., et. al., Dynamic CT imaging of volumetric changes in pulmonary nodules correlates with physical measurements of stiffness. Radiotherapy Oncology, 2017 February, 122(2): 313-318. | Billy W. Loo, and Peter G. Maxim, Stanford University School of Medicine, Stanford, CA, USA | Cancer, Lung cancer | Non-invasive scanning | XRAY | Rat | Ami HTX | 2017 | Lung cancer: non-invasive screening, X-ray guided tumor cell injections, pulmonary nodule stiffness or deformability, correlation with pulmonary nodule (PN) volume ratio, technologies used: PN stiffness: Young’s modulus using atomic force microscopy, PN volume ratio: respiratory-gated MicroCT, Probe: None, X-ray imaging | | cancer lung-cancer | non-invasive-scanning |
| Lahiji, S.F., et. al., Transcutaneous implantation of valproic acid-encapsulated dissolving microneedles induces hair regrowth, Biomaterials, 2018. | Hyungil Jung, Yonsei University, Seoul, Republic of Korea | Androgenetic Alopecia | Hair regrowth model | FLI | Mouse | Lago X | 2018 | Hair regrowth: androgenetic alopecia, valproic acid (VPA)-encapsulating dissolving microneedle (DMN-VPA), micro wounding, subdermal, Probe: FITC | | androgenetic-alopecia | hair-regrowth-model |
| Kumar, G.D., et. al., Modified coring tool designs reduce iceberg lettuce cross-contamination. Journal of Food Protection, 2019, 82(3): 454–462. | Sadhana Ravishankar, University of Arizona, Tucson, AZ, USA | Plant Pathogens | Crop harvesting | BLI | Coring machine, Iceberg Lettuce | Ami HT | 2019 | Crop harvesting: Coring tool, minimizing bacterial cross-contamination, food safety, lettuce, Salmonella Newport serotype, Probe: luciferase | | plant-pathogens | crop-harvesting |
| Kimbrough, C.W., et. al., Targeting acidity in pancreatic adenocarcinoma: Multispectral optoacoustic tomography detects pH-low insertion peptide probes in vivo. Clinical Cancer Research: An official journal of the American Association for Cancer Research, 2015, 21(20): 4576–4585. | Lacey R. McNally, University of Louisville, Louisville, KY, USA | Cancer, Pancreatic Cancer | pH-low insertion peptides as TME-specific probe | BLI, FLI | Mouse | Ami HTX | 2015 | pH-low insertion peptides (pHLIP): pH-sensitive membrane intercalation, target acidic tumor microenvironment (TME), pancreatic ductal adenocarcinoma, human pancreatic cancer cell line S2VP10, multispectral optoacoustic tomography (MSOT), Probes: HiLyte Fluor 750 C2 maleimide (AnaSpec) conjugated to pH insensitive pHLIP K7 or to ph sensitive pHLIP V7 | | cancer pancreatic-cancer | ph-low-insertion-peptides-as-tme-specific-probe |
| Kimbrough, C.W., et. al., Orthotopic pancreatic tumors detected by optoacoustic tomography using Syndecan-1. Journal of Surgical Research, 2015, 193(1): 246–254. | Lacey R. McNally, University of Louisville, Louisville, KY, USA | Cancer, Pancreatic Cancer | FLI and MSOT Detection Assay | BLI, FLI | Mouse | Ami HTX | 2015 | Localizing orthotopic pancreatic adenocarcinoma: syndecan-1, human pancreatic cancer cell line S2VP10, multispectral optoacoustic tomography (MSOT), ICG as negative control dye, Probes: Syndecan-1, ICG, luciferase | | cancer pancreatic-cancer | fli-and-msot-detection-assay |
| Ke, B., et. al., In vivo bioluminescence imaging of cobalt accumulation in a mouse model. Analytical Chemistry, 2018, 90: 4946-4950. | Lupei Du and Minyong Li, Shandong University, Jinan, Shandong, China | Cobalt, Trace Element Nutrient Tracking | BLI Detection Assay | BLI | Mouse | Lago X | 2018 | Trace element nutrient tracking: Cobalt, Cobalt Bioluminescent Probe 1 (CBP-1), luciferin-cage conjugate , Probe: luciferase | | cobalt trace-element-nutrient-tracking | bli-detection-assay |
| Kang, Y.Y., et. al., Byakangelicin as a modulator for improved distribution and bioactivity of natural compounds and synthetic drugs in the brain, Phytomedicine, 2019, 62. | Hyejung Mok, Konkuk University, Seoul, Republic of Korea | Central Nervous System, Inflammation | Combinatorial drug synergistic efficacy | FLI | Mouse | Lago X | 2019 | Byakangelicin enhances the pharmacokinetics (blood brain barrier (BBB) penetration) of various drugs: umbelliferone (Umb), curcumin (Cur), and doxorubicin (Dox), Probe: Green Fluorophore | | central-nervous-system inflammation | combinatorial-drug-synergistic-efficacy |
| Kaemmer, C.A., et. al., Development and comparison of novel bioluminescent mouse models of pancreatic neuroendocrine neoplasm metastasis. Nature Portfolio: Scientific Reports, 2021, 11. | Dawn E. Quelle, University of Iowa, Iowa City, IA, USA | Cancer, Pancreatic Cancer | Pancreatic neuroendocrine neoplasms (pNENs), preclinical BLI model development | BLI | Mouse | Ami HT | 2021 | Pancreatic neuroendocrine neoplasms (pNENs): preclinical BLI model development, biodistribution, liver metastases, lung metastases, Probe: luciferase | | cancer pancreatic-cancer | pancreatic-neuroendocrine-neoplasms-pnens preclinical-bli-model-development |
| Johnson, J., et. al., Genomic profiling of a Hepatocyte growth factor-dependent signature for MET-targeted therapy in glioblastoma. Journal of Translational Medicine, 2015, 13(1): 306. | Qian Xie, Van Andel Research Institute, Grand Rapids, MI, USA | Cancer, Glioblastoma | Genomic profiling of MET-inhibitor sensitive glioblastoma cell lines | BLI | Mouse | Ami HT | 2015 | Genetic profiling of MET-inhibitor sensitive glioblastomas: elevated Hepatocyte Growth Factor (HGF) expression correlated with MET-inhibitor efficacy, Probe: luciferase | | cancer glioblastoma | genomic-profiling-of-met-inhibitor-sensitive-glioblastoma-cell-lines |
| Jeffery, J.J., et. al., Autocrine inhibition of the c-fms proto-oncogene reduces breast cancer bone metastasis assessed with in vivo dual-modality imaging. Experimental Biology and Medicine, 2014, 239(4): 404–413. | Setsuko K Chambers, University of Arizona Cancer Center, Tucson, AZ, USA | Breast Cancer, Cancer | Breast cancer bone metastases prevention | BLI, uCT | Mouse | Ami HT | 2014 | Breast Cancer Bone Metastases Prevention: c-fms proto-oncogene, osteolysis, autocrine, polyclonal anti-human c-fms antibody, Probe: luciferase | | breast-cancer cancer | breast-cancer-bone-metastases-prevention |
| Ippen, F.M., et. al., The dual PI3K/mTOR pathway inhibitor GDC-0084 achieves antitumor activity in PIK3CA-mutant breast cancer brain metastases. Clinical Cancer Research: An official journal of the American Association for Cancer Research, 2019, 25(11): 3374–3383. | Priscilla K. Brastianos, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA | Breast Cancer, Cancer | Breast cancer brain metastases treatment | BLI | Mouse | Ami HTX | 2019 | Breast Cancer Brain Metastases Treatment: GDC-0084, dual PI3K/mTOR inhibitor , Probe: luciferase | | breast-cancer cancer | breast-cancer-brain-metastases-treatment |
| Hudson, S.V., et. al., Targeted noninvasive imaging of EGFR-expressing orthotopic pancreatic cancer using multispectral optoacoustic tomography. Cancer Research, 2014, 74(21): 6271–6279. | Lacey R. McNally, University of Louisville, Louisville, KY, USA | Cancer, Pancreatic Cancer | Pancreatic cancer specific NIR fluorescent probe | BLI, FLI | Mouse | Ami HTX | 2014 | Pancreatic Cancer: Epidermal growth factor receptor (EGFR), targeted by EGF-CF750 conjugate probe, detected by FLI or multispectral optoacoustic tomography (MSOT), luciferase-transduced S2VP10L tumors, Probes: CF-750, luciferase | | cancer pancreatic-cancer | pancreatic-cancer-specific-nir-fluorescent-probe |
| Huang, J., et. al., An activatable near-infrared chromophore for multispectral optoacoustic imaging of tumor hypoxia and for tumor inhibition, Theranostics, 2019, 9(24):7313-7324. | Fang Zeng, and Shuizhu Wu, South China University of Technology, Guangzhou, China | Cancer, Tumor Hypoxia | Hypoxia-specific NIR activateable probe conjugated to DNA-binding therapeutic | FLI | Mouse | Ami HT | 2019 | Tumor hypoxia: Liposome encapsulated, combined NIR fluorophore probe and DNA-binding therapeutic, nitrogen mustard, are activated by hypoxia-associated reductases , Probe: NR-NH2, an amino-containing xanthene fluorophore | | cancer tumor-hypoxia | hypoxia-specific-nir-activateable-probe-conjugated-to-dna-binding-therapeutic |
| Huang, D., et. al., Polymeric nanoparticles functionalized with muscle-homing peptides for targeted delivery of phosphatase and tensin homolog inhibitor to skeletal muscle. Acta Biomaterialia, 2020. | Feng Yue, and Shihuan Kuang, Purdue University, West Lafayette, IN , USA | Duchenne muscular dystrophy (DMD) | Muscle growth and repair | FLI | Mouse | Ami HT | 2020 | Duchenne muscular dystrophy (DMD) treatment: Normalizing muscle growth and repair, phosphatase and tensin homolog (PTEN) inhibitors, nanoparticles (NP), muscle homing peptide, M12, Probe: Alexa Fluor 488 | | duchenne-muscular-dystrophy-dmd | muscle-growth-and-repair |
| Hipsch, M., et. al., Sensing stress responses in potato with whole-plant redox imaging. Plant Physiology, 2021. | Shilo Rosenwasser, The Hebrew University of Jerusalem, Rehovot, Israel | Radical Oxygen Species (ROS) | Plant Oxidative Stress | FLI | Potato Plant (cv. Desiree) | Ami HT | 2021 | Radical oxygen species (ROS) production during Environment Stress Conditions: Hydrogen peroxide levels detected by chloroplast associated, glutathione redox potential, in turn, measured by redox sensitive GFP (roGFP2), in Solanum tuberosum potato, environmental stressors tested: temperature, light exposure, drought, Probe: roGFP2 | | radical-oxygen-species-ros | plant-oxidative-stress |
| Heo, R., et. al., Dextran sulfate nanoparticles as a theranostic nanomedicine for rheumatoid arthritis, Biomaterials, 2017. | Jae Hyung Park, Sungkyunkwan University, Suwon, Republic of Korea | Autoimmunity, Rheumatoid Arthritis | Therapeutic delivery by Nanoparticles | FLI | Mouse | Ami HT | 2017 | Rheumatoid Arthritis Treatment: dextran sulfate nanoparticles, methotrexate therapeutic, taken up by activated macrophages (via scavenger receptor class A endocytosis) at sites of RA joint inflammation, Probe: FPR-675 | | autoimmunity rheumatoid-arthritis | therapeutic-delivery-by-nanoparticles |
| Henry, M.D., et. al., Comparison of high sensitivity BLI imaging systems for ultra-weak signal applications. (Conference poster). | 1-Michael D. Henry, and 2-Michael D. Cable, 1-University of Iowa Carver College of Medicine, Iowa City, IA, USA, and 2-Spectral Instruments Imaging, Tucson, AZ, USA | Bioluminescent Imaging | Signal calibration across field of view (FOV) | BLI | Mouse | Ami HTX | na | Bioluminescent Imaging Devices: normalizing light collection efficiency across FOV, light optics, flat field corrections in Spectral Instruments Imaging vs. IVIS® systems, Probe: luciferase | | bioluminescent-imaging | signal-calibration-across-field-of-view-fov |
| Hardy, S.D. et. al., Regulation of epithelial-mesenchymal transition and metastasis by TGF-beta, P-bodies, and autophagy. Oncotarget, 2017, 8(61): 103302–103314. | Michael K. Wendt and Robert L. Geahlen, Purdue University, West Lafayette, IN , USA | Breast Cancer, Cancer | Molecular Biology of Epithelial Mesenchymal transition (EMT) | BLI | Mouse | Ami HT | 2017 | Breast Cancer: Processing bodies (P-bodies), stress associated post-transcriptional mRNA metabolism, cleared by autophagy, but accumulates with expression of transforming growth factor-beta (TGF-β) leading to epithelial-mesenchymal transition (EMT), DDX6 inhibition blocks P-body formation and EMT, Probe: luciferase | | breast-cancer cancer | molecular-biology-of-epithelial-mesenchymal-transition-emt |
| Han, N., et. al., Development of surface-variable polymeric nanoparticles for drug delivery to tumors. Molecular Pharmaceutics, 2017, 14(5): 1538–1547. | Yoon Yeo, Purdue University, West Lafayette, IN , USA | Nanoparticle delivery systems | NP targeting of tumor microenvironment (TME) | FLI | Mouse | Ami HT | 2017 | Tumor targeting Nanoparticles (NPs): coated with amidated TAT peptides, accumulate in acidic tumor microenvironment (TME), due to TAT carboxy residue formation ("activation"), mouse in vivo studies fail due to premature activation not seen in vitro, Probe: DiR | | nanoparticle-delivery-systems | np-targeting-of-tumor-microenvironment-tme |
| Haber, Z., et. al., Resolving diurnal dynamics of the chloroplastic glutathione redox state in Arabidopsis reveals its photosynthetically-derived oxidation, The Plant Cell, 2021. | Shilo Rosenwasser, The Hebrew University of Jerusalem, Rehovot, Israel | Radical Oxygen Species (ROS) | Plant Oxidative Stress | FLI | Arabidopsis | Ami HT | 2021 | Plant oxidative stress: ROS, chloroplastic glutathione redox potential (chl-EGSH), light-dark, dark-light transitions, protection of photosystem I (PSI) and phoytosystem II PSII) from photoinhibition, Probe: roGFP2 | | radical-oxygen-species-ros | plant-oxidative-stress |
| Feng, X., et. al., Responsive Fluorescence Probe for Selective and Sensitive Detection of Hypochlorous Acid in Live Cells and Animals. Chemistry, An Asian Journal, 2018, 10.1002/asia.201800957 | 1-Qingtao Meng, and 2-Run Zhang, 1-University of Science and Technology, Liaoning, Anshan, China and 2-The University of Queensland, Brisbane, Australia. | Radical Oxygen Species (ROS) | ROS detection by fluorescence imaging | FLI | Zebra Fish; Mouse | Ami HT | 2018 | Reactive Oxygen Species (ROS) Detection: HOCl detection, zebra and mouse models, novel fluorophore, Probe: PQI | | radical-oxygen-species-ros | ros-detection-by-fluorescence-imaging |
| Feng, X., et. al., Dying glioma cells establish a proangiogenic microenvironment through a caspase 3 dependent mechanism. Cancer Letters, 2017, 385: 12–20. | 1-Chuan-Yuan Li, 2-Ling Tian, and 2-Qian Huang, 1-Duke University Medical Center, Durham, NC, USA, and 2-Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China | Cancer, Glioblastoma | re-angiogenesis, Tumor recurrence | BLI | In vitro; Mouse | Ami HTX | 2017 | Glioblastoma: Tumor recurrence, re-angiogenesis, Caspase 3 dependent, molecular mechanisms, COX-2/PGE2, VEGF-A, Probe: luciferase | | cancer glioblastoma | re-angiogenesis tumor-recurrence |
| Fan, Q., et. al., Modulation of pericytes by a fusion protein comprising of a PDGFRβ-antagonistic affibody and TNFα induces tumor vessel normalization and improves chemotherapy, Journal of Controlled Release, 2019, 302: 63-78. | Jingqiu Cheng, and Xiaofeng Lu, West China Hospital, Sichuan University, Chengdu, China | Cancer, Sarcoma | Tumor vascular normalization | FLI | Mouse | Lago X | 2019 | Sarcoma: tumor vascular normalization, cancer-targeted therapy, pericytes, TNFα, affibody, doxorubicin, Probe: CF-750 succinimidyl ester | | cancer sarcoma | tumor-vascular-normalization |
| England, C.G., et. al., Detection of phosphatidylcholine-coated gold nanoparticles in orthotopic pancreatic adenocarcinoma using hyperspectral imaging. PloS one, 2015, 10(6). | Hermann B. Frieboes, University of Louisville, Louisville, KY, USA | Cancer, Pancreatic Cancer | Nanoparticle intra-tumoral penetration | BLI | Mouse | Ami HTX | 2015 | Gold Nanoparticle (NP) Intratumoral Transport Barriers: interstitial fluid pressure (IFP), chaotic and dense extracellular matrix, acidic micro-environment in orthotopic pancreatic adenocarcinoma (PAC). Penetration improved by: reduced NP size, phosphatidylcholine-coating (vs. PEG coating); detection by hyperspectral imaging, Probe: luciferase | | cancer pancreatic-cancer | nanoparticle-intra-tumoral-penetration |
| England, C.G., et. al., X-ray skeleton imaging in conjunction with bioluminescence imaging does not alter pancreatic tumor. (Conference poster). | Lacey R. McNally, University of Louisville, Louisville, KY, USA | XRAY Imaging | Animal safety, Oncogenesis | BLI, XRAY | Mouse | Ami HTX | na | Safety of in vivo X-Ray Imaging: Oncogenesis, Pancreatic Cancer, Probe: luciferase | | xray-imaging | animal-safety oncogenesis |
| Elattar, S., et. al., The tumor secretory factor ZAG promotes white adipose tissue browning and energy wasting. The FASEB Journal, 2018, 32: 4727–4743. | Ande Satyanarayana, Augusta University, Augusta, Georgia, USA | Cachexia | Cachexia molecular, therapeutic target | FLI | Mouse | Ami HT | 2018 | Cachexia: tissue-wasting syndrome characterized by inflammation, hyper metabolism, increased energy expenditure, and anorexia. Zinc-α2-glycoprotein (ZAG) as a therapeutic target, beige adipocyte, Ebf2, Prdm16, Ucp1, Probe: Td-Tomato | | cachexia | cachexia-molecular therapeutic-target |
| Dykstra, M., et. al., In vitro and in vivo studies of cerenkov luminescence imaging. (Conference poster). | Michael Dykstra, Grand Valley State University, Allendale, MI, USA, and Van Andel Research Institute,Grand Rapids, MI, USA | Cerenkov Imaging | Cerenkov Imaging with Optical Imager | BLI | Mouse | Ami HT | na | Cerenkov Luminescence Imaging: positron, positron emission tomography (PET), Probe: 18F-FDG | | cerenkov-imaging | cerenkov-imaging-with-optical-imager |
| Dassie, J.P., et. al., Targeted inhibition of prostate cancer metastases with an RNA aptamer to prostate specific membrane antigen (PSMA). Molecular Therapy, 2014, 22(11): 1910–1922. | Paloma H. Giangrande, University of Iowa, Iowa City, IA, USA | Cancer, Pancreatic Cancer | Metastases Inhibition | BLI, FLI | Mouse | Ami HT | 2014 | Pancreatic Cancer, bone metastases: Tumor-targeting smart drugs, RNA Aptamer (A9g), inhibitor of prostate-specific membrane antigen (PSMA), Probes: luciferase, IRDye 800CW | | cancer pancreatic-cancer | metastases-inhibition |
| Dann, T., et. al., Anatase titanium dioxide imparts photoluminescent properties to PA2200 commercial 3D printing material to generate complex optical imaging phantoms. Materials, 2021, 14(7): 1813. | W. Matthew Leevy, University of Notre Dame, 1234 N Notre Dame Avenue, South Bend, IN, USA | Imaging Phantoms | Photoluminescent 3D-print phantoms | PL | Optical Phantoms | Ami HT | 2021 | Optical Imaging Phantoms: Selective laser sintering, 3D-printed, nylon 12 and titanium dioxide (TiO2), photoluminescence, rat anatomical phantom, derenzo phantom, Probe: TiO2 | | imaging-phantoms | photoluminescent-3d-print-phantoms |
| Connolly, R.J., et. al., Development of a catheter-based applicator for immuno-oncology. (Conference Poster). | Robert H. Pierce, OncoSec Medical Incorporated, San Diego, CA, USA | Cancer, Melanoma | Immunotherapy | BLI | Mouse | Lago | na | Melanoma: Immuno-oncology, immunotherapy, electroporation, catheter, intratumoral delivery of cytokine encoding plasmids, IL-12, Probe: luciferase | | cancer melanoma | immunotherapy |
| Chaurasiya, S., et. al., Toward comprehensive imaging of oncolytic viroimmunotherapy, Molecular Therapy: Oncolytics, 2021 | Susanne Warner, City of Hope National Medical Center, Duarte, CA, USA | various | Viral oncolytic therapy | BLI | various | Lago X | 2021 | Viral Oncolytic Therapy: tumor micro environment (TME), viroimmunotherapy, Probe: luciferase | | various | viral-oncolytic-therapy |
| Buchakjian, M.R., et al., Development of a Tongue Carcinoma Model Using Real-Time In Vivo Molecular Monitoring. (Conference Poster). | Michael D. Henry, University of Iowa Carver College of Medicine, Iowa City, IA, USA | Cancer, Sarcoma | Oncogenesis | BLI | Mouse | Ami HTX | na | Tongue Carcinoma: adenovirus CRE recombinase, TP53/PTEN knockout / luc knockin, Probe: luciferase | | cancer sarcoma | oncogenesis |
| Buchakjian, M.R., et. al., A Trp53 fl/fl Pten fl/fl mouse model of undifferentiated pleomorphic sarcoma mediated by adeno-Cre injection and in vivo bioluminescence imaging. PLoS One, 2017, 12(8). | Michael D. Henry and Christopher S. Stipp, University of Iowa Hospitals & Clinics, and University of Iowa Carver College of Medicine, Iowa City, IA,USA | Cancer, Sarcoma | Oncogenesis | BLI | Mouse | Ami HTX | 2017 | Sarcoma: adenovirus CRE recombinase, TP53/PTEN knockout / luc knockin, Probe: luciferase | | cancer sarcoma | oncogenesis |
| Borin, T.F., et. al., HET0016 decreases lung metastasis from breast cancer in immune-competent mouse model. PloS One, 2017, 12(6). | Ali S. Arbab, Augusta University, Augusta, GA, USA | Breast Cancer, Cancer | Immunotherapy | BLI | Mouse | Ami HTX | 2017 | Breast Cancer: T41 cells, lung metastases, immunosuppression, myeloid-derived suppressor cells (MDSC), tumor microenvironment (TME), selective inhibitor of 20-HETE synthesis, Probe: luciferase | | breast-cancer cancer | immunotherapy |
| Bhutiani, N., et. al., Detection of microspheres in vivo using multispectral optoacoustic tomography. Biotech Histochemistry, 2017, 92(1): 1–6. | Lacey R. McNally, University of Louisville, Louisville, KY, USA | Nanoparticles (NPs) | NIRF labeling of NPs | FLI | Mouse | Ami HT | 2017 | Microsphere particle delivery system: liver, optoacoustic tomography, Probe: IR-780-iodide | | nanoparticles-nps | nirf-labeling-of-nps |
| Bahrami, A.J., et. al., A novel approach for endoscopic gene transfer. (Conference Poster), 2017. | Robert H. Pierce,OncoSec Medical Incorporated, San Diego, CA, USA | Cancer, Melanoma | Immunotherapy | BLI | Mouse | Lago | 2017 | Melanoma: intratumoral gene therapy, electroporation, IL-12, Probe: luciferase | | cancer melanoma | immunotherapy |
| Au, K.M., et. al., Pretargeted delivery of PI3K/mTOR small-molecule inhibitor-loaded nanoparticles for treatment of non-Hodgkin’s lymphoma. Science Advances, 2020, 6: 1-12. | Steven I. Park, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA | Cancer, Non-Hodgkin's Lymphoma | B Cell Receptor transduction cascade regulation | FLI | Mouse | Ami HT | 2020 | Non-Hodgkin's Lymphoma (NHL): B Cell receptor transduction cascade, dual PI3K/mTOR inhibitor, delivered by antibody-predirected nanoparticles, , Probe: Cy5 | | cancer non-hodgkins-lymphoma | b-cell-receptor-transduction-cascade-regulation |
| Alizadeh, D., et. al., INFɣ is Critical for CAR T cell mediated myeloid activation and induction of endogenous immunity. Cancer Discovery, 2021, 11(9): 2248-2265. | Christine E. Brown, City of Hope National Medical Center, Duarte, CA, USA | Cancer, Glioblastoma | Immunotherapy | BLI | Mouse | Ami HT | 2021 | Glioblastoma (GBM): chimeric antigen receptor (CAR) T cells, IFNγ, tumor microenvironment, myeloid cells, endogenous memory T cells, Probe: luciferase | | cancer glioblastoma | immunotherapy |
| Alizadeh, D., et. al., Doxorubicin eliminates myeloid-derived suppressor cells and enhances the efficacy of adoptive T-cell transfer in breast cancer. Cancer research, 2014, 74(1): 104–118. | Nicolas Larmonier, University of Arizona, Tucson, Arizona, USA | Breast Cancer, Cancer | Immunotherapy | BLI | Mouse | Ami HT | 2014 | Breast Cancer: Myeloid-derived suppressor cells (MDSC), immunotherapy, doxorubicin, Th1, Th17, Probe: luciferase | | breast-cancer cancer | immunotherapy |
| Alexander, M.S., et. al., A model for the detection of pancreatic ductal adenocarcinoma circulating tumor cells. Journal of Biological Methods, 2018, 5(3): 1-7. | Michael D. Henry, and Joseph J. Cullen, University of Iowa College of Medicine, Iowa City, USA | Cancer, Pancreatic Cancer | Metastases formation | BLI | Mouse | Ami HT | 2018 | Pancreatic ductal adenocarcinoma (PDAC): circulating tumor cells (CTCs), metastasis, orthotopic implantation, Probes: luciferase, GFP | | cancer pancreatic-cancer | metastases-formation |
| Achyut, B.R., et. al., Chimeric mouse model to track the migration of bone marrow derived cells in glioblastoma following anti-angiogenic treatments. Cancer Biology & Therapy, 2016, 17(3): 280–290. | Ali S. Arbab, Georgia Regents University, Augusta, GA, USA | Cancer, Pancreatic Cancer | Antiangiogenic resistance | FLI | Mouse | Ami HT | 2016 | Glioblastoma (GBM): tumor antiangiogenic resistance, tumor microenvironment, bone marrow, vascular endothelial growth factor (VEGF), Probe: GFP | | cancer pancreatic-cancer | antiangiogenic-resistance |
| Achyut, B.R., et. al., Bone marrow derived myeloid cells orchestrate antiangiogenic resistance in glioblastoma through coordinated molecular networks. Cancer Letters, 2015, 369(2): 416–426. | Ali S. Arbab, Georgia Regents University, Augusta, GA, USA | Cancer, Glioblastoma | Antiangiogenic resistance | FLI | Mouse | Ami HT | 2015 | Glioblastoma (GBM): tumor antiangiogenic resistance, tumor microenvironment, bone marrow, vascular endothelial growth factor (VEGF), Probes: GFP, mCherry Red | | cancer glioblastoma | antiangiogenic-resistance |
